Intravital Microscopy System

Two-Photon and Confocal Microscopy System for Live Animal Imaging

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Models & Specs

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Features

System Overview

Intravital Microscopy(IVM) is an all-in-one two-photon and/or confocal microscopy system designed and optimized for longitudinal imaging of live animal models in vivo. 

This state-of-art equipment has been designed around ease-of-use and augmented throughput as a next-generation core technology for biologists and translational scientists to elucidate the underlaying mechanism of every biological phenomena at tissue and cellular level. Confocal IVM systems enable optical sectioning of in vivo tissue via rejection of out-of-focus fluorescence light coming from the background tissue which will result in images with high contrast and quality. Two-photon IVM systems are equipped with lasers which use longer-wavelength near-infrared (NIR) fs-pulse excitation capable of deep tissue imaging as well as label-free, non-linear multi-harmonic generation imaging (SHG, THG).

IVM upright systems are equipped with fully-integrate body temperature regulation and anesthesia inlet/outlet into the stage.

The system also includes state-of-the-art live tissue motion compensation for improved image quality, along with ultrafast imaging speeds to track fast-moving imaging targets within the tissue. The system may be configured for confocal or two-photon microscopy modules.

Features & Benefits

Intravital Microscopy(IVM) is an all-in-one two-photon and/or confocal microscopy system designed and optimized for longitudinal imaging of live animal models in vivo.

Integrated heated animal stage and physiological controller

Ensures animal well-being throughout the imaging session, and consistency between animals within a study

Motion compensation function

Provides enhanced image quality on imaging dynamic organs, automatically compensating for the effects of respiratory motions and brain pulses

User-friendly ergonomics and user interface

Allows for ease of use, and reproducible results by experts and non-experts

Integration inhalation anesthesia inlet/outlet

Enables connection to an external inhalation anesthesia machine. Compatible with all universal anesthesia machines.

Animal stabilizing holders and hardware

Quick, secure stabilization of the animal on the stage for time-laps and longitudinal imaging, minimizing motion artefacts

Fast to ultrafast scanning

Allows users to track the movement of several cells in vivo to better understand the biological processes being examined

4-color simultaneous imaging

Multiplexity and simultaneous monitoring of various labelled elements within the tissue

4D Imaging

The software allows for acquisition of 3D stacks of moving objects over time and rendering it as a 4D image

Models & Specifications

IVM-MS3

Compact Two-Photon Laser Unit
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IVM-CMS3

Confocal Laser Unit Compact Two-Photon Laser Unit
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IVM-C3

Confocal Laser Unit
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IVM-M3

Tunable Two-Photon Laser Unit
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IVM-CM3

Confocal Laser Unit Tunable Two-Photon Laser Unit
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Accessories & Add-ons (All Models)

Accesories Add-on
- Inhalation Anaesthesia Accessories
- Optional high (60fps) or ultra-high (100fps) speed scanning module
- Optional objective lens set for two-photon or confocal imaging
- In vivo imaging window chamber sets
- Cranial imaging (bone, bone marrow, dura-matter, brain)
- Dorsal skin imaging (skin and subcutaneous tumors)
- Thoracic imaging (heart and lung)
- Abdominal imaging (internal abdominal organs)
- Pancreas imaging
- Fluorescent labeling agents, vascular dyes and conjugated anti-bodies
- Variable Emission Filter (Optional)
- 6 or 2 emission filter can be mounted on each of four detectors
ScanHead
Scanner
Polygonal mirror (Fast axis scanning, Max. 66 kHz)
Galvano scanner (Slow axis scanning, Max. 200 ㎲ /step)
Imaging Head
Objectives
Polygonal mirror (Fast axis scanning, Max. 66 kHz)
Galvano scanner (Slow axis scanning, Max. 200 ㎲ /step)
Image
FOV

Pixel Resolution

Imaging Speed
100 x 100 ㎛ ² – 10 x 10 mm²

Max. 2,048 x 2,048 pixels
Standard : 30 fps @ 512 x 512 pixels

(Optional) High Speed : 60 fps @ 512 x 512 pixels
(Optional) Ultra High Speed : 100 fps @ 512 x 512 pixels
In Vivo Animal Stage
3D Stage
Travel Range : 50,000 x 50,000 x 75,000 ㎛ (XYZ)
Micromanipulation (Max. 0.2 ㎛ resolution)
3-axis independent control with Jog Dial & S/W
Animal Motion Compensation
F4D In Vivo Imaging Motion Compensation
XY motion compensation:
Averaged image acquisition with motion artifact compensation
Z motion compensation:
Image-based sample Z position adjustment for long-term intravital microscopic imaging
& sample tracking (Feedback-loop automatic stage control)
T motion compensation:
Image-based image XY position adjustment for long-term intravital microscopic imaging & sample tracking (Feedback-loop automatic stage control)
Combination of above three compensation for 4D in vivo motion compensation
Controllable by IVM Engine software
Additional In Vivo Modules
Live Animal Maintenance Unit


In Vivo Imaging Chamber SET








Inhalation Anesthesia System

Body Temp. Monitoring & Feedback Heater Control including tablet PC
4CH Rectal Probe, Body Plate Heater, Thermometer Sensor & Cover Glass Heater

Standard Dorsal Skinfold Chamber SET
Lung Imaging Chamber SET
Cranial Window SET
Abdominal Imaging Window SET
Pancreas Imaging Window SET
Mammary Imaging Window SET
Heart Imaging Window SET

Rodent Animal Inhalation Anesthesia System
Anesthesia Mask and Connections
Engine & Studio Software
Image Display



In Vivo Imaging Mode

Independent 4 single channel display (RGBA channel)
Overlay channel display (Selection among RGBA channel)


Mosaic imaging (XY), Z-stack imaging (Z), Time-lapse imaging (T)
Time-lapse imaging at Multi-position (T- M),
Time-lapse & Z-stack imaging (TZ),
Time-lapse & Z-stack imaging at Multi-position (TZ- M)

IVIM Tech Imaging Gallery

Limph Node
Lung
Bone Marrow
Skin
Cancer Xenograft
Cancer Metastasis
Cancer Drug Delivery
Drug Distribution

Past Webinars - IVIM Tech

Publications & Articles