The Prospect T1 is an innovative high-frequency ultrasound system designed specifically for in vivo preclinical imaging in small animals such as mice and rats. This compact and cost-effective tablet-based system provides high-resolution images (up to 30 µm) and advanced capabilities to monitor changes in hemodynamics and observe anatomical structures in real-time.
With its standard ultrasound capabilities, such as B-mode, M-mode, Color/Power Doppler, Pulsed Wave Doppler, Tissue Doppler, and Contrast imaging, the Prospect T1 offers an array of imaging options for a wide range of applications.
Additionally, the system offers access to the RAW digital RF data, making it possible to generate quantitative results and perform offline data analysis.
The included scanning platform is equipped with a heated mouse or rat-sized platform and integrated physiological monitoring, making it easier to position the animal and acquire high-quality images.
Three different single-element probes are available to choose from, depending on the animal to be imaged and the anatomical targets. The ergonomic design of the animal handling apparatus ensures easy positioning and cleaning between imaging subjects, while the analytical tools and measurement packages help to speed up the process of generating results.
Features & Benefits
The Prospect T1, a cost-effective high-frequency preclinical ultrasound system, can be used within the laboratory. No more need for a core imaging facility.
Standard Imaging Modes
- Pulsed Wave, Tissue, Color, Power Doppler
- Contrast Mode
(Linear and non-linear/harmonic)
Probes & Platforms
Animal Handling Platforms:
- Mouse Table
- Rat Table
Comprehensive Measurement Packages
Users can quickly perform the measurements, compile data reports, and generate quantifiable outputs from images and spectrograms.
Analysis may be done on the system, or an offline analysis workstation.
ECG Gated Kilohertz Visualization (EKV)
This mode allows users to acquire B-mode images of the heart with up to 400fps; these images provide exquisite detail of cardiac motion and may be used with any of the standard measurement tools.
RAW Data and Import Scripts
Researchers who want to look at the digital RF data from any mode can easily access it and import it into third-party software for further analysis.
Compact & Small Footprint
Tablet-based acquisition computer and compact scanning platform.
The system can easily be moved and does not need to reside in a core facility.
If needed, the system can fit within a biosafety cabinet to help researchers who may be working with immunocompromised animals or those with other concerns.
Simplified Image Guided Injection Mount
With the probe and injection mount connected, once the needle and imaging plane are aligned, the user can move the probe/needle as needed to perform the injection.
Add-on Hardware/Software Options
- 3D Motor, including software for volume measurements
- Image Guided Injection Mount, with software image guide
- Shear Wave Elastography/ Acoustic Radiation Force push probe and software integration
Intuitive Workflow with Touch Screen
Preclinical researchers can quickly start their studies, acquire and store images from the various modes available, review their data and perform their required measurements.
(Keyboard and mouse also included)
Non-linear Contrast Agent Imaging (First Harmonic)
This increases sensitivity to microbubble contrast agents without requiring reference subtraction for linear contrast agent imaging.
This improves signal sensitivity and the risk of motion artifacts within the images.is available using both the 20MHz and 40MHz probes.
Mouse Left Ventricle in Long Axis – B-Mode
Mouse Carotid Artery – B-Mode
Mouse E15 Embryo – B-Mode
Mouse Mammary Fat Pad Tumor – 3D B-Mode
ECG gated kilohertz visualization (EKV)
Mouse Left Ventricle in Long Axis – B-Mode with 30fps
ECG gated kilohertz visualization (EKV)
Mouse Left Ventricle in Long Axis – EKV with 120fps, now possible up to 400fps
Mouse Left Ventricle in Short Axis – M-Mode
Mouse Aortic Arch – Color Doppler
Mouse Subcutaneous Tumor – Power Doppler
Pulsed Wave (PW) Doppler
Mouse Mitral Valve Inflow – PW Doppler
Mouse Mitral Valve Annulus – Tissue Doppler
Mouse subcutaneous tumor – Non-linear contrast agent imaging with regions of interest drawn
Mouse subcutaneous tumor – Non-linear contrast agent imaging Time vs. Intensity plots for drawn regions of interest
Shear Wave Elastography
Shear wave elastography is a technique used to study and quantify the mechanical and elastic properties of various tissues including liver, breast, tendons, muscles, etc. An acoustic radiation force is generated by a secondary, non-imaging, element mounted on the side of the imaging probe. The software is used to program the pulse sequence to generate the shear wave; the wave will propagate faster through stiffer tissues, as well as along the axis of various musculoskeletal structures. The analysis software generates a colored elastogram which is overlaid upon a B-mode image. Tissues with focal or diffuse lesions may be identified using this technique.
Propagation of Shear Wave through mouse liver
Shear Wave Elastogram showing focal lesion in mouse liver
Models & Specifications
PB506e – Center Frequency 50MHz (30-60MHz)
PB406e – Center Frequency 40MHz (20-50MHz)
PB207e – Center Frequency 20MHz (10-30MHz)
Mouse Platform 17.2 x 14.2cm
Rat Platform 28.3 x 18.2cm
Standard, Advanced & Optional
Pulsed Wave / Tissue / Color / Power Doppler
Contrast Mode (Linear and Non-Linear/Harmonic
RAW data available for all modes
Accessories & add-ons
3D Motor and Acquisition/Analysis Software
The 3D motor connects to the imaging platform to precisely move the animal bed with imaging subject under the probe. The acquisition software allows the user to select the distance the motor will travel and the step size. The acquired imaging slices are then reconstructed into a 3D image. The visualization and analysis software allows the user to move through the acquired image, view it as a 3D surface, and make volume measurements.
Mammary Fat Pad Tumor in Mouse – 3D Image
Mammary Fat Pad Tumor in Mouse – 3D Volume = 263mm3
Mouse Hind Limb Muscle – 3D Image
Mouse Hind Limb Muscle – 3D Volume = 97.6mm3
Prospect T1 Imaging Gallery
The Prospect T1 is routinely used for cardiovascular imaging of a variety of small animal models, including mice and rats.
Real-time images are provided of the heart and a wide variety of blood vessels.
The system can be used to assess both systolic and diastolic function in the heart; as well to image and assess flow through any number of blood vessels, including the aorta, pulmonary, carotid, renal, splenic, and femoral arteries etc. Veins, including the vena cava and portal veins may also be assessed.
Imaging may be performed in utero, as early as day 1 after delivery, all the way through adulthood. Cardiovascular function may be of interest as disease progresses, perhaps induced by surgical intervention, transgenic mutation, or systemic treatment with a therapy or toxic compound. Ultrasound imaging is non-invasive, so the same measurements may be taken repeatedly over the course of a longitudinal study.
Mouse Left Ventricle in Long Axis in B-Mode
Mouse Left Ventricle in Short Axis in M-Mode with Measurements to Calculate Systolic Function
Mouse Mitral Valve Inflow in PW Doppler with Measurements to Calculate Diastolic Function
Mouse Mitral Annulus in Tissue Doppler with Measurements to Assess Diastolic Function
Caption – Mouse Aortic Arch in Color Doppler
Mouse Carotid Artery in B-Mode
Mouse Pulmonary Artery in PW Doppler to Assess RV Outflow
Publications & Articles
Ursolic acid prevents doxorubicin‐induced cardiac toxicity
Ursolic acid prevents doxorubicin‐induced cardiac toxicity in mice through eNOS activation and inhibition
Synergistic inhibitory effects on hepatocellular carcinoma
Synergistic inhibitory effects on hepatocellular carcinoma with recombinant human adenovirus Aspp2 and oxaliplatin
The Mouse Abdominal Imaging Guide
Mouse Abdominal Imaging Guide: Imaging the Liver, Hepatic Vessels, Portal Veins and Gallbladder