RWD Fiber Photometry System

R810- Dual Color Multichannel Fiber Photometry System

Detecting activity change of neurons in the central nervous system in real-time, the fiber photometry system sums up the overall fluorescence of neurons to express overall activity without reflecting activity of individual neurons. Based on the reference channel of 410 nm wavelength, RWD Fiber Photometry System adopts 470 nm and 560 nm dual-wavelength excitation, reducing the background noise effectively. The significance of the reference channel lies in increasing self-control, reducing spontaneous fluorescence, eliminating signal artifacts caused by animal’s intense exercise and fiber winding, expressing two kinds of proteins at the same site, detecting levels of calcium change in different groups of neurons, and true recording and analysis of dual-color fluorescence signals.

Features:

  • Two kinds of LED excitation light sources at 410 & 470 nm, of which 410 is the reference channel, allows for a more accurate fluorescence signal acquisition.
  • It can support up to 9 channels, suitable for high-throughput calcium signal acquisition or simultaneous recording of multiple neural nucleus groups.
  • When the external event is marked, the time automatically inputs to achieve a synchronous recording with behavioral video, and the external stimulus time synchronization accuracy is up to 10ms.
  • The software has four pre-set fluorescence mode options, which can be applied to different experimental application scenarios.
  • High-performance and low noise CMOS camera can collect calcium fluorescence signals of multiple channels at up to 300 frames per second (fps).
  • 4 input ports support a variety of external signal input and automatic marking while 4 output ports support TTL signals to trigger external equipment.

Technical Specifications:

Frame Rate of CMOS 0~300 FPS
 sCMOS camera resolution 2048 * 2048 px
 Exposure time setting range 1~100 ms
 Fluorescence band 500-550 nm
Central wavelength of excitation light 410 nm, 470 nm
 LED power adjustable percentage 0~100%
 410nm LED Output Power Range 0~100 μW
 470nm LED Output Power Range 0~100 μW