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You are here:///Oxygen Monitors – Oxford Optronix
Oxygen Monitors – Oxford Optronix2018-05-17T19:37:04+00:00

OxyLite™

Third generation, single-channel dissolved oxygen (pO2) and temperature monitor

  • Oxygen (pO2) & temperature monitor

  • In vivo & in vitro applications

  • Fully ‘plug and play’; no calibration procedures

  • Single-channel monitor

Product Brochure
See product reviews on SelectScience
  • Oxford Optronix - OxyLite System Front
  • Oxford Optronix - OxyLite System Back
  • Oxford Optronix - OxyLite System Connector

OxyLite™ Pro / Pro XL

Third generation, multi-channel dissolved oxygen (pO2) and temperature monitors with touch-screen

  • Oxygen (pO2) & temperature monitor

  • In vivo & in vitro applications

  • Fully ‘plug and play’; no calibration procedures

  • 2 or 4-channel monitors

  • Enhanced productivity & features

  • Touch-screen display

Product Brochure
See product reviews on SelectScience
  • Oxford Optronix - OxyLite Pro System Front
  • Oxford Optronix - OxyLite Pro System Back
  • Oxford Optronix - OxyLite Pro System Connector

Overview

The survival of tissues and organs relies on an adequate supply of oxygen. The measurement of tissue oxygen tension (ptiO2) provides a direct measurement of the balance between oxygen supply (by the blood) and metabolic oxygen consumption (by the tissue), i.e. a readout of oxygen availability at the cellular level. This is in contrasts to spectroscopy (NIRS) techniques, which merely describe haemoglobin oxygenation status.

OxyLite™ oxygen monitors will be of interest to life scientists wishing to directly and continuously measure dissolved oxygen in the normal physiological as well as hypoxic ranges, both in experimental in vivo models, or in any number of in vitro applications.

Since 1998, when Oxford Optronix pioneered the commercialization of fibre-optic oxygen micro-sensor technology, the OxyLite™ brand has become established the world over in biomedical, boasting in excess of 500 peer-reviewed journal citations and hundreds of units sold worldwide.

2013 saw the culmination of a decade and half of continuous R&D with the launch of the third-generation of tissue oxygen monitors. Blending contemporary design, true plug and play convenience and the very latest in opto-electronic technology, OxyLite™ Pro systems provide what is simply the most advanced, accurate and reliable tissue oxygen monitoring platform on the market.

Oxford Optronix oxygen sensors leverage state-of-the-art optical fluorescence technology intended for the quantitative measurement of oxygen partial pressure (pO2) and temperature in tissues, physiological fluids, cell cultures and other in vitro applications. This technology offers key advantages over devices based on polarographic technology, making OxyLite™ oxygen monitors much easier to use and ideally suited to oxygen measurements in the typical physiological range, as well as incredibly sensitive under conditions of hypoxia.

Applications include cancer biology, angiogenesis, stroke and brain injury, vital organ and muscle tissue monitoring, flap monitoring, ophthalmology, wound healing, as well as dissolved oxygen monitoring in cell culture, bioreactors etc.

Statement of intended use:
OxyLite™ and OxyLite™ Pro are intended for laboratory, industrial and research use only.
OxyLite™ and OxyLite™ Pro are NOT intended for use in human subjects/patients.

Oxford Optronix - Made In Britain

Key Benefits

The easiest-to-use dissolved oxygen monitors in the world!

No calibration procedures, no sensor drift, no obligatory PC/software interface, integrated temperature compensation, oxygen partial pressure displayed in absolute units of mmHg. OxyLite™ oxygen monitors are simply the most user-friendly oxygen monitors available today, period!

Absolute units of dissolved oxygen

The fluorescence-based technique employed by OxyLite™ oxygen monitors provides an absolute measurement of dissolved oxygen in mmHg or kPa. In vivo this provides a direct readout of oxygen availability to cells and tissue, in contrast to blood oxygen saturation assessment (pulse oximetry), which merely describes the haemoglobin oxygenation status of blood.

Sensitivity and accuracy

Third-generation opto-electronics provide unmatched sensitivity, stability and accuracy in the physiologically relevant pO2 range (0 – 200 mmHg) and under conditions of hypoxia (0 – 15 mmHg).

No oxygen consumption

Zero oxygen consumption at the point of measurement, thus innately suitable for continuous and absolute oxygen sensing, even under conditions of extreme hypoxia.

Microchip sensors

‘EEPROM’ technology embedded within the purpose-designed connectors provides unsurpassed ease of use and user convenience by completely eliminating all calibration procedures. Simply ‘plug and go’!

Touch-screen display and interface

OxyLite™ Pro models feature a touch-sensitive, high-contrast, 140º viewing-angle screen, which displays real-time data in both digital and graphical (trace) formats, and provides access to instrument/user settings.

Built-in temperature compensation

Automatic temperature compensation of the pO2 measurement through optional, integrated temperature sensors.

Versatility

Full support for both in vivo (tissue) pO2 applications AND measurement of dissolved oxygen in vitro (e.g. hypoxic cell culture, tissue constructs, bioreactors and more).

Minimally invasive for in vivo applications

Oxygen sensor diameters range from approximately 230 µm – 750 µm to suit a host of tissue monitoring applications and cause minimal tissue disruption in use.

MRI compatibility

Dedicated sensor types provide support and compatibility for magnetic resonance imaging (selected sensor types only).

Multi-channel productivity

The OxyLite™ Pro is available as a 2-channel model or, uniquely, as a 4-channel device (OxyLite™ Pro XL) capable of driving up to 4 sensors simultaneously. This provides the flexibility to suit all needs, including the simultaneous monitoring of oxygen from multiple tissue sites (e.g. comparison of pathological versus control tissue sites), or multiple in vitro samples.

Upgradeable

The OxyLite™ Pro 2-channel model can be factory upgraded to the 4-channel model (OxyLite™ Pro XL).

Single-sensor multi-parameter monitoring

OxyLite™ oxygen monitors have been specifically developed for use with OxyFlo™ blood flow monitors. The combination of these two fibre optic measurement systems provides simultaneous tissue blood flow and oxygenation data from each ‘triple’ parameter combined sensor.

USB digital output

The entire range of Oxford Optronix tissue monitoring devices include a dedicated USB output that supports direct streaming of real-time recordings to a PC running the popular LabChart® Pro* charting software by ADInstruments. After installing a complimentary Add-on, LabChart will automatically identify the specific type and model of Oxford Optronix monitor and pre-load all the necessary configuration and channel settings, providing the ultimate in ‘plug and play’ convenience. For researchers wishing to make multi-parameter measurements, the Add-on supports multiple Oxford Optronix devices at the same time. Data acquisition and recording just got a whole lot easier!

ADInstruments LabChart

*LabChart version 8.0.4 or later required; PC/Windows only

Analogue outputs

Continuous data recording to PC or Mac platforms is also supported via standard analogue data outputs offering compatibility with third party data recording solutions.

2-year product warranty

Oxford Optronix monitors are now provided with a comprehensive 2-year manufacturer’s warranty, covering defects in material or in workmanship. An optional extended warranty package, providing up to 6 years of ‘peace of mind’ cover, including preventative maintenance servicing is available at the time of purchase.

Data Example

A Model of Muscle Fatigue in Mouse

A single, combined oxygen/temperature sensor was used to continuously measure hind leg muscle tissue pO2 and temperature during repeated phases of contraction stimulation. The upper trace shows pO2 in units of mmHg; the lower trace shows temperature in units of degrees C.

Click the data set image to view full screen.

Oxford Optronix - OxyLite - Muscle Fatigue in Mouse

User Testimonials

“Our group has successfully applied OxyLite needle probes to measure changes in tissue oxygenation in response to drug treatment. OxyLite has allowed us to collect important mechanistic data to identify the mechanism of these drug treatments in laboratory animals. We greatly appreciate having a calibrated method like the OxyLite probe technology available that allows the collection of accurate, real-time physiological data from live animals.”
– Dr. Thies Schroeder, Duke University Medical Center, Department of Radiation Oncology, Durham NC, United States

“One particularly useful ability of the OxyLite [is the ability] to observe the oxygen measurements alongside absorbance readings, plotting both the oxygen and enzyme kinetics. This would never have been possible with a standard Clarke Electrode. I am genuinely impressed with the OxyLite, its range of applications and its ease of use. I would be more than happy to recommend it to the scientific community.”
 Dr. Catherine Hoare, Hope Hospital, Manchester, UK

“In our use with transplantable tumors we’ve found the OxyLite to be stable, sensitive and versatile..” “..the ability to measure dynamic changes in oxygen tension is a powerful feature.”
– Dr Dennis B. Leeper, Past Director and Founder, Division of Experimental Radiation Oncology, Thomas Jefferson University, Philadelphia, USA

“I use the BF/OT/E PO2 E-series sensor to measure PaO2 in vivo in invertebrates during external environmental fluctuations in PO2. Due to the accuracy and reliability of the PO2 probes we can design experiments that provide real time measurements of PO2 during changes in environmental variables and more accurately link this to behavioural responses. During the experimental set up the staff at Oxford Optronix were always on hand to help out with any technical queries and even visited the laboratory to check out the experimental set up and ensure the probes were working. I would recommend Oxford Optronix to anyone with an interest in measuring PO2 and temperature in marine science.”
– 
Dr Elizabeth Morgan, National Oceanography Centre, University of Southampton, UK

“Your equipment has taken me to some weird and wonderful places and contributed to about a dozen original articles over the last decade.”
– Dr. Roger Evans, Monash University, Department of Physiology, Melbourne, Australia

Applications and Literature Citations

OxyLite™ monitors are employed the world over with an extensive citation record across a wide range of research applications summarized in the categories below.  Click any abstract to view online, or click DOWNLOAD CITATIONS PDF for a complete list of all known literature citations.

Selected Citations

Physiology/Ischaemia

Mahling M, Fuchs K, Thaiss WM, Maier FC, Feger M, Bukala D, Harant M, Eichner M, Reutershan J, Lang F, Reischl G, Pichler BJ and Kneilling M,  A Comparative pO2 Probe and [18F]-Fluoro-Azomycinarabino-Furanoside ([18F]FAZA) PET Study Reveals Anesthesia-Induced Impairment of Oxygenation and Perfusion in Tumor and Muscle. PLoS One (2015); 10(4), e0124665 [LINK]

Instrument cited: OxyLite™ 2000

Rafferty AR, Evans RG, Scheelings TF and Reina RD,  Limited oxygen availability in utero may constrain the evolution of live birth in reptiles. Am Nat (2013); 181(2), 245-53 [LINK]

Instrument cited: OxyLab™ 2000

Rausch ME, Weisberg S, Vardhana P and Tortoriello DV,  Obesity in C57BL/6J mice is characterized by adipose tissue hypoxia and cytotoxic T-cell infiltration. Int J Obes (Lond)(2008); 3, 451-63 [LINK]

Instrument cited: OxyLite™ 2000

Methodology/Validation

Penjweini R, Kim MM, Finlay JC and Zhu TC, Investigating the impact of oxygen concentration and blood flow variation on photodynamic therapyProc SPIE Int Soc Opt Eng (2016), 9694 [LINK]

Instrument cited: OxyLite™

Wen, B., Urano, M., Humm, J. L., Seshan, V. E., Li, G. C. and Ling, C. C, Comparison of Helzel and OxyLite Systems in the Measurements of Tumor Partial Oxygen Pressure (pO2)Radiat. Res. (2008); 168, 67-75 [LINK]

Instrument cited: OxyLite™ 4000

Elas M, Ahn KH, Parasca A, Barth ED, Lee D, Haney C and Halpern HJ, Electron paramagnetic resonance oxygen images correlate spatially and quantitatively with OxyLite oxygen measurementsClin Cancer Res (2006); 12, 4209-17 [LINK]

Instrument cited: OxyLite™ 2000

Tumour/Cancer

Luan X, Guan YY, Lovell JF, Zhao M, Lu Q, Liu YR, Liu HJ, Gao YG, Dong X, Yang SC, Zheng L, Sun P, Fang C, Chen HZ, Tumor priming using metronomic chemotherapy with neovasculature-targeted, nanoparticulate paclitaxelBiomaterials (2016); 95, 60-73 [LINK]

Instrument cited: OxyLite™

Corroyer-Dulmont A, Chakhoyan A, Collet S, Durand L, MacKenzie ET, Petit E, Bernaudin M, Touzani O and Valable S, Imaging Modalities to Assess Oxygen Status in GlioblastomaFront Med (Lausanne)(2015); 2, 57 [LINK]

Instrument cited: OxyLite™ 2000

Collet G, Lamerant-Fayel N, Tertil M, El Hafny-Rahbi B, Stepniewski J, Guichard A, Foucault-Collet A, Klimkiewicz K, Petoud S, Matejuk A, Grillon C, Jozkowicz A, Dulak J, Kieda C, Hypoxia-regulated overexpression of soluble VEGFR2 controls angiogenesis and inhibits tumor growthMol Cancer Ther (2014); 13(1):165-78 [LINK]

Instrument cited: OxyLite™ 2000

In Vitro Applications

Tiwari A, Wong CS, Nekkanti LP, Deane JA, McDonald C, Jenkin G and Kirkland MA, Impact of Oxygen Levels on Human Hematopoietic Stem and Progenitor Cell Expansion. Stem Cells Dev. 2016 Aug 18. [Epub ahead of print] [LINK]

Instrument cited: OxyLite™ Pro

You JO, Rafat M, Almeda D, Maldonado N, Guo P, Nabzdyk CS, Chun M, LoGerfo FW, Hutchinson JW, Pradhan-Nabzdyk LK, Auguste DT, pH-responsive scaffolds generate a pro-healing response. Biomaterials (2015); 57, 22-32 [LINK]

Instrument cited: OxyLab p02™ 

Handley MG, Medina RA, Mariotti E, Kenny GD, Shaw KP, Yan R, Eykyn TR, Blower PJ and Southworth R, Cardiac hypoxia imaging: second-generation analogues of 64Cu-ATSM. J Nucl Med (2014); 55(3), 488-94 [LINK]

Instrument cited: OxyLab p02

Ophthalmology

Murali K, Kang D, Nazari H, Scianmarello N, Cadenas E, Tai YC, Kashani A and Humayun M, Spatial Variations in Vitreous Oxygen Consumption. PLoS One (2016); 11(3), e0149961 [LINK]

Instrument cited: OxyLab p02

Lange CA, Stavrakas P, Luhmann UF, de Silva DJ, Ali RR, Gregor ZJ and Bainbridge JW, Intraocular oxygen distribution in advanced proliferative diabetic retinopathy. Am J Ophthalmol (2011); 152(3), 406-412 [LINK]

Instrument cited: OxyLab p02™ 

Siegfried CJ, Shui YB, Holekamp NM, Bai F and Beebe DC, Oxygen distribution in the human eye: relevance to the etiology of open-angle glaucoma after vitrectomy. Invest Ophthalmol Vis Sci. (2010); 51(11), 5731-8 [LINK]

Instrument cited: OxyLab p02

Plastic Surgery/Wound Healing

Ponticorvo A1, Taydas E, Mazhar A, Scholz T, Kim HS, Rimler J, Evans GR, Cuccia DJ, Durkin AJ, Quantitative assessment of partial vascular occlusions in a swine pedicle flap model using spatial frequency domain imaging. Biomed Opt Express (2013); 4(2):298-306 [LINK]

Instrument cited: OxyLite 2000

Schlaudraff KU, Pepper MS, Tkatchouk EN, Ehrenburg I, Alizadeh N, Montandon D and Pittet B, Hypoxic preconditioning increases skin oxygenation and viability but does not alter VEGF expression or vascular density. High Alt Med Biol (2008); 9(1), 76-88 [LINK]

Instrument cited: OxyLite 4000

Russell JA, Conforti ML, Connor NP and Hartig GK, Cutaneous tissue flap viability following partial venous obstruction. Plast Reconstr Surg. (2006); 117(7), 2259-66 [LINK]

Instrument cited: OxyLite 2000

Vital Organs/Shock

Cezar CA, Roche ET, Vandenburgh HH, Duda GN, Walsh CJ and Mooney DJ, Biologic-free mechanically induced muscle regeneration. Proc Natl Acad Sci USA (2016); 113(6):1534-9 [LINK]

Instrument cited: OxyLab p02

Muir WW, Del Rio CL, Ueyama Y, Youngblood BL, George RS, Rausch CW, Lau BS, Hamlin RL, Dose-Dependent Hemodynamic, Biochemical, and Tissue Oxygen Effects of OC99 following Severe Oxygen Debt Produced by Hemorrhagic Shock in Dogs. Crit Care Res Pract. (2014); 864237 [LINK]

Instrument cited: OxyLite 2000

Zhang S, Han C-H, Chen X-S, Zhang M, Xu L-M, Zhang J-J and Xia Q, Transient Ureteral Obstruction Prevents against Kidney Ischemia/Reperfusion Injury via Hypoxia-Inducible Factor (HIF)-2 alpha Activation. PLoS One (2012); 7(1), e29876 [LINK]

Instrument cited: OxyLab p02

Cerebral/Stroke/MCAO

Bakhsheshi MF, Moradi HV, Stewart EE, Keenliside L and Lee TY, Brain Cooling With Ventilation of Cold Air Over Respiratory Tract in Newborn Piglets: An Experimental and Numerical Study. IEEE J Transl Eng Health Med (2015); 3, 1500108 [LINK]

Instrument cited: OxyLab p02

Bouzat P, Millet A, Boue Y, Pernet-Gallay K, Trouve-Buisson T, Gaide-Chevronnay L, Barbier EL, Payen JF., Changes in brain tissue oxygenation after treatment of diffuse traumatic brain injury by erythropoietin. Crit Care Med. (2013); 41(5):1316-24 [LINK]

Instrument cited: OxyLite 2000

Dunn JF, Wu Y, Zhao Z, Srinivasan S and Natah SS, Training the brain to survive stroke. PLoS One (2012); 7(9): e45108 [LINK]

Instrument cited: OxyLite 2000

Specifications for OxyLite and OxyLite Pro

GENERAL
Dimensions OxyLite: 95mm (H) x 290mm (W) x 260mm (D)
OxyLite Pro: 150mm (H) x 310mm (W) x 280mm (D)
Weight OxyLite: 2kg / 4.5lbs
OxyLite Pro: 4kg / 9lbs
Operating temperature 10 – 30°C
Operating humidity 0 – 70% (non-condensing)
Power requirements OxyLite: VAC 100-240V, 50-60Hz, 30W max
OxyLite Pro: External PSU (100-240V/50-60Hz), 40W max
Fuse rating OxyLite: 2 x T1.6A
OxyLite Pro: Not applicable
Number of sensors (channels) supported OxyLite: 1
OxyLite Pro: 2
OxyLite Pro XL: 4
Display OxyLite: 40 character alphanumeric OLED
OxyLite Pro: High contrast 800 x 480 LCD touch-screen
Instrument configuration OxyLite: Via function button at rear
OxyLite Pro: Via touch-screen display
Analogue voltage outputs OxyLite: 2 x male BNC connectors (2 outputs)
OxyLite Pro: 1 x 15-pin D-type (4 outputs)
OxyLite Pro XL: 2 x 15-pin D-type (8 outputs)
LED excitation wavelength 525nm
Luminescence wavelength 650nm
PERFORMANCE
Mode of operation Luminescence decay lifetime (pO2) / T-type thermocouple or manual user input (temperature)
Measurement units (displayed) mmHg or kPa / ºC
Measurement range 0 – 200mmHg; 0 – 26.6kPa / 0 – 50ºC
Measurement resolution 0.1mmHg / 0.1ºC
Measurement accuracy Oxygen:
±0.7mmHg (0 – 7mmHg)
±10% of reading (7 – 150mmHg)
±15% of reading (150 – 200mmHg)Temp: ±0.2ºC
Measurement response time < 20s (standard oxygen sensor) / < 2s (temperature)
Measurement sampling rate 1Hz (user definable on OxyLite Pro models)
Measurement acquisition time 1s
Measurement averaging 5s, rolling
Display update interval (numerical) 2s (5s rolling averaged)
Validated temp compensation range 10ºC – 45ºC
Sensor identification Automatic; integrated connector EEPROM
Sensor calibration Factory pre-calibration (unique per sensor); valid 6 months
Sensor shelf-life 2 years from factory calibration (used or unused)
Sensor longevity 48 hours accumulated usage at default sampling rate
Analogue data output range 0 – 5V (0 – 200mmHg / 0 – 50ºC) (default)
Analogue data output rate 1Hz

Frequently Asked Questions

What physiological parameters does OxyLite™ measure?

Oxford Optronix oxygen monitors are intended to measure the partial pressure of oxygen (pO2) in aqueous conditions (i.e. conditions of 100% relative humidity). The oxygen partial pressure is also often referred to as ‘dissolved oxygen’ and is very much distinct from the measurement of haemoglobin oxygen saturation using spectroscopic techniques (e.g. a pulse oximetry).  OxyLite™ monitors also optionally provide a real-time readout of temperature from the sensor tip.

How do pO2 measurements differ from oxygen saturation measurements?

The fluorescence-based technique employed by Oxford Optronix oxygen monitors provides an absolute measurement of dissolved oxygen in mmHg or kPa. In tissue monitoring applications this provides a direct readout of the balance between oxygen supply (by the blood) and oxygen consumption (by tissue/cell metabolism), i.e. a readout of oxygen availability to cells and tissue. This contrasts to blood oxygen saturation assessment (pulse oximetry), which merely describes the haemoglobin oxygenation status.

What makes the OxyLite™ unique?

A number of key features make Oxford Optronix oxygen monitors stand out. Most notable are unmatched ease of use thanks to proprietary ‘EEPROM’ connectors, quality and measurement accuracy.  OxyLite™ also uniquely supports simultaneous measurement of oxygen and blood perfusion in tissues when used in a stack configuration with its OxyFlo™ blood flow monitor counterpart.

What are the typical applications for the OxyLite™?

Oxford Optronix oxygen monitors have proved popular in applications covering such disciplines as tumour research, cerebral monitoring, free-flap/pedicle-flap transfer surgery, transplantation surgery and vital organ monitoring, peripheral vascular disease research and in a variety of specialist in vitro applications including hypoxic cell culture, tissue engineering and bioreactor monitoring.

How do Oxford Optronix oxygen sensors work?

Oxford Optronix oxygen sensors are based on fluorescence quenching and fibre-optic technology. Short pulses of LED light are transmitted along the fibre optic sensor to excite a platinum-based fluorophore bonded to the sensor tip. The resulting emission of fluorescent light, quenched by the presence of oxygen molecules is detected by the instrument. The instrument measures the lifetime of fluorescence, which is inversely proportional to the concentration of dissolved oxygen and is interpreted to provide an absolute value for oxygen in mmHg or kPa.

What are the advantages of oxygen sensing using fluorescence technology?

The novel fluorescence-based technology employed in Oxford Optronix sensors and monitors offers several key advantages over devices based on polarographic electrodes. These include an absence of oxygen consumption (allowing continuous oxygen measurement, even in hypoxic conditions), factory sensor pre-calibration, calibration stability and maximal sensitivity in the physiological oxygen range (0 – 200 mmHg). A number of Oxford Optronix fibre-optic sensors also offer MRI-compatibility.

What procedures are required to calibrate Oxford Optronix oxygen sensors?

None! Oxford Optronix oxygen sensors are shipped factory pre-calibrated thereby eliminating time consuming pre- or post-calibration procedures. The calibration information (unique per sensor) is stored on an EEPROM chip within the sensor connector and is read by the monitor within seconds upon connection. Oxford Optronix oxygen sensors are therefore fully ‘plug and go’.

What is the measurement range of Oxford Optronix oxygen sensors?

Oxford Optronix oxygen sensors are intended for the measurement of dissolved oxygen (pO2) in the typical physiological range. The officially supported range is 0 – 200 mmHg (0 – approx. 25% oxygen). Maximum sensitivity is observed between 0 – 150 mmHg.

What are Oxford Optronix oxygen sensors made of and how large are they?

All Oxford Optronix oxygen microsensors are constructed from optical fibres with an outside diameter of 230 microns. Sensors are provided in a number of formats, including minimally invasive ‘bare-fibre’ format (with and without integrated temperature sensor), in a robust needle-encased format (approx. diameter 650 microns), or in a specialist format for large area sensing (typically in large animal models) (approx. diameter 650 microns). Optical fibres are protected in PVC or silicone sleeving and are typically 2.5 m in length. SEE THE SENSORS TAB for a list of available oxygen sensor types.

What are the advantages of fibre-optic sensors?

The use of fibre-optic sensors allows them to be minimally invasive, to be factory recalibrated, to offer MRI-compatibility, as well as to be light weight and physically flexible.

Do Oxford Optronix oxygen monitors support the use of multiple sensors at the same time?

Yes, the OxyLite™ Pro is a two-channel monitor, while the OxyLite™ Pro XL is a four-channel monitor capable of simultaneously driving and reading from up to 4 oxygen sensors simultaneously. This allows simultaneous sampling from several samples in vitro, or the collection of physiological data from up to four separate tissue sites at the same time. The latter may be useful when comparing pathological versus control tissue sites. The ability to monitor from more than one tissue site also provides unique possibilities for studying spatial tissue oxygen variability, or, conversely, can be useful in mitigating for natural tissue oxygen variability by allowing multi-site signal averaging.

Are Oxford Optronix sensors supplied sterile?

No. While Oxford Optronix manufactures their oxygen sensors in a clean room facility, they are not supplied sterile. However, oxygen sensors can be disinfected using 70% IMS or ethanol.  Please Note: neither Oxford Optronix oxygen sensors, nor oxygen monitors possess CE or FDA regulatory approval for use in human subjects.

Can the OxyLite™ or the OxyLite™ Pro models be used for in vitro oxygen measurements?

Yes! Oxford Optronix oxygen monitors and sensors are ideally suited for in vitro applications where expected oxygen levels fall within the supported range of the instrument (0 – 200 mmHg, or 0 – approx. 25% oxygen). Their small size and fibre-optic construction mean that Oxford Optronix oxygen sensors are particularly well suited to applications such as hypoxic cell culture, tissue engineering and/or bioreactor surveillance.

Are Oxford Optronix oxygen sensors suited to measuring arterial oxygen (paO2) or venous oxygen (pvO2)?

Yes, provided oxygen concentrations fall within the 0 – 200 mmHg supported dynamic range, oxygen can be measured continuously from whole blood in situ. Extended exposure may result in clotting around the sensor tip, depending on the presence or absence of anticoagulant (e.g. Heparin).

Why does Oxford Optronix offer oxygen sensors with integrated temperature?

Fluorescence-based oxygen sensing shows a small degree of sensitivity to temperature changes. For optimal accuracy, Oxford Optronix oxygen monitors can therefore use a continuous temperature input (provided by the thermocouple) to automatically compensate the oxygen measurement. The temperature reading is also available as an output where temperature is a desirable parameter in its own right.

What is the supported temperature range for Oxford Optronix oxygen sensors?

The supported temperature range is 0 – 50 degrees Celsius. Effective pO2 signal temperature compensation has been tested over the range 25-44 degrees Celsius.

What is the sampling volume of Oxford Optronix pO2 sensors?

The surface area of the tip of Oxford Optronix fibre-optic oxygen sensors is estimated to be approx. 0.25 mm2. In a typical tissue this surface area may correspond to direct exposure to around 1000 cells. On the basis that several cell layers are likely to contribute to the dissolved oxygen diffusing in and out of the sensor tip, and assuming typical cell volume (size) and tissue cell densities, the volume of tissue sampled by the oxygen sensors in vivo is estimated to be in the region of 0.5 – 1 mm3.

Do Oxford Optronix oxygen monitors possess regulatory approval for clinical use?

No. At this time Oxford Optronix oxygen monitors and sensors do NOT possess CE or FDA regulatory approvals for use on human subjects.

Do you offer on-site demonstrations of Oxford Optronix instruments?

Yes, depending on geographical location we will try to arrange for an on-site demonstration of Oxford Optronix products by an experienced product specialist. Ideally, this will involve a full simulation of your application in order to maximize the benefit of the demonstration. On-site demonstrations are usually free of charge and can typically be arranged at 2 – 8 weeks notice. Alternatively, it is possible to arrange a limited trial of select monitors and sensors of interest.

Oxygen Sensors for OxyLite™ and OxyLite™ Pro

A wide selection of oxygen sensors are available to suit both physiological (in vivo) monitoring and in vitro applications. Sensors featuring integrated thermocouples support automatic temperature-compensation of oxygen readings.

Oxygen sensors are supplied in sealed Tyvek® pouches.

Oxford Optronix - Oxylite - Oxygen sensors for OxyLite™ and OxyLite™ Pro

Bare-Fibre Type Oxygen Sensors

Oxygen-Only Bare-Fibre Sensor
Product Code: ‘NX-BF/O/E’

Oxford Optronix - OxyLite - Product code 'NX-BF/O/E'

Flexible, minimally invasive sensor suitable for localized oxygen measurements from virtually any tissue type, or for in vitro dissolved oxygen applications.

  • Tissue placement via introducer (e.g. Angiocath®)
  • T-90 response time approx. 20 s
  • Manual temperature compensation required
  • Removable/adjustable Luer
  • MRI compatible

Dimensions:

  • Tip diameter: approx. 250 µm
  • Exposed tip fibre length: 6 cm
  • Total sensor length: 2.5 m

Reusability: Limited

NOTE: THIS SENSOR TYPE IS ALSO AVAILABLE IN 8 M LENGTH FOR MRI APPLICATIONS

Oxygen/Temperature Bare-Fibre Sensor
Product code ‘NX-BF/OT/E’

Oxford Optronix - OxyLite - Product code 'NX-BF/OT/E'

Flexible, minimally invasive sensor suitable for localized combined oxygen/temperature measurements from virtually any tissue type, or for in vitro dissolved oxygen applications.

  • Tissue placement via introducer (e.g. Angiocath®)
  • T-90 response time approx. 20 s
  • Automatic temperature compensation
  • Removable/adjustable Luer

Dimensions:

  • Tip diameter: approx. 350 µm
  • Exposed tip fibre length: 6 cm
  • Total sensor length: 2.5 m

Reusability: Limited

Needle Type Oxygen Sensors

Oxygen-Only Needle-Encased Sensor
Product code ‘NX-NP/O/E’

Oxford Optronix - OxyLite - Product code 'NX-NP/O/E'

23G surgical steel-encased sensor featuring greater physical robustness, suitable for localized oxygen measurements from virtually any tissue type, or for in vitro dissolved oxygen applications. Oxygen detection via lateral ‘windows’ distal from the sensor tip. Larger tissue sampling area/volume than bare-fibre type sensors (1 mm²).

  • Tissue placement: directly, or via introducer (e.g. Angiocath®)
  • T-90 response time approx. 3 min
  • Manual temperature compensation required
  • Fixed/potted Luer

Dimensions:

  • Needle diameter: approx. 650 µm
  • Exposed needle length: 6 cm
  • Total sensor length: 2.5 m

Reusability: Limited

Large Area Type Oxygen Sensors

Oxygen-Only Large Area Sensor (1 mm²)
Product code ‘NX-LAS-1/O/E’

Oxford Optronix - OxyLite - Product code 'NX-LAS-1/O/E'

Sensor tip shielded within a short 23G surgical steel cage, oxygen detection via lateral ‘windows’, 1 mm² tissue sampling area/volume. Suitable for localized oxygen measurements from virtually any tissue type, or for in vitro dissolved oxygen applications.

  • Tissue placement via introducer (e.g. Angiocath®)
  • T-90 response time – approx. 3 min
  • Manual temperature compensation required

Dimensions:

  • Tip diameter: approx. 650 µm
  • Tip ‘cage’ length: 5 mm
  • Total sensor length: 2.5 m

Reusability: Limited

Oxygen-Only Large Area Sensor (8 mm²)
Product code ‘NX-LAS-8/O/E’

Oxford Optronix - OxyLite - Product code 'NX-LAS-8/O/E'

Sensor tip shielded within an extended 23G surgical steel cage, oxygen detection via lateral ‘windows’, very large tissue sampling area/volume (8 mm²) for area-averaged tissue oxygen measurements.

  • Tissue placement via introducer (e.g. Angiocath®)
  • T-90 response time – approx. 3 min
  • Manual temperature compensation required

Dimensions:

  • Tip diameter: approx. 650 µm
  • Tip ‘cage’ length: 12 mm
  • Total sensor length: 2.5 m

Reusability: Limited

Oxygen/Temperature Large Area Sensor (8 mm²)
Product code ‘NX-LAS-8/OT/E’

Oxford Optronix - OxyLite - Product code 'NX-LAS-8/OT/E'

Combined oxygen/temperature sensor tip shielded within an extended 23G surgical steel cage, oxygen detection via lateral ‘windows’, very large tissue sampling area/volume (8 mm²) for area-averaged tissue oxygen/temperature measurements.

  • Tissue placement via introducer (e.g. Angiocath®)
  • T-90 response time – approx. 3 min
  • Automatic temperature compensation

Dimensions:

  • Tip diameter: approx. 650 µm
  • Tip ‘cage’ length: 12 mm
  • Total sensor length: 2.5 m

Reusability: Limited

Implantable Oxygen Sensors

Implantable Bare-Fibre Type
Oxygen Sensor

Product code ‘NX-CI/BF/O/E’

Oxford Optronix - OxyLite - Product code 'NX-CI/BF/O/E'

Flexible, minimally invasive oxygen sensor suitable for chronic tissue implantation.

  • Tissue placement via introducer
    (e.g. Angiocath®)
  • T-90 response time approx. 20 s
  • Manual temperature compensation required
  • NX-CI ADAPTER required (see accessories)
  • Supplied with calibration on iButton device
  • MRI compatible

Dimensions:

  • Tip diameter: approx. 250 µm
  • Exposed tip fibre length: 60 mm (default); 2 mm minimum
  • Ferrule length: 10 mm

Reusability: Intended for single use

Implantable Large Area Type
Oxygen Sensor (1 mm²)

Product code ‘NX-CI/LAS-1/O/E’

Oxford Optronix - OxyLite - Product code 'NX-CI/LAS-1/O/E'

Sensor tip shielded within a short 23G surgical steel cage, oxygen detection via lateral ‘windows’, 1 mm² tissue sampling area/volume.

  • Tissue placement via introducer
    (e.g. Angiocath®)
  • T-90 response time – approx. 3 min
  • Manual temperature compensation required

Dimensions:

  • Tip diameter: approx. 650 µm
  • Tip ‘cage’ length: 5 mm
  • Length from ferrule: 60 mm
  • Ferrule length: 10 mm

Reusability: Intended for single use

Implantable Large Area Type
Oxygen Sensor (8 mm²)

Product code ‘NX-CI/LAS-8/O/E’

Oxford Optronix - OxyLite - Product code 'NX-CI/LAS-8/O/E'

Sensor tip shielded within an extended 23G surgical steel cage, oxygen detection via lateral ‘windows’, very large tissue sampling area/volume (8 mm²) for area-averaged tissue oxygen measurements.

  • Tissue placement via introducer
    (e.g. Angiocath®)
  • T-90 response time – approx. 3 min
  • Manual temperature compensation required

Dimensions:

  • Tip diameter: approx. 650 µm
  • Tip ‘cage’ length: 12 mm’
  • Length from ferrule: 60 mm
  • Ferrule length: 10 mm

Reusability: Intended for single use

Optional Accessories

Value ADC

12-input analogue data acquisition module.
Oxford Optronix value data recording proposition, supplied with WinDAQ software by Dataq Instruments.

Oxford Optronix - OxyLite - Value ADC

NX-CI Adapter

Oxford Optronix specialist implantable (‘CI’ type) oxygen sensors require this 2.5 m connecting adapter.
The adapter is re-usable and features an ‘iButton’ receiver pad which is used to transmit the calibration data for the attached sensor.
All CI type sensors are supplied with an iButton device carrying the unique calibration data for that sensor.

Oxford Optronix - OxyLite - NX-CI ADAPTER

BNC data cables

1 m BNC data cables for single channel OxyLite™ and OxyFlo™ monitors.
For data connection to third-party data recorder (e.g. PowerLab™ or Value ADC).

Oxford Optronix - OxyLite - BNC data cables

BNC Adapter cable for pro models

1 m high density 15-pin D-connector to 4 BNC data adapter cable for OxyLite™ Pro and OxyFlo™ Pro monitors.
For data connection to third-party data recorder (e.g. PowerLab™ or Value ADC).

Oxford Optronix - OxyLite - BNC adapter cable for Pro models

Measuring Tissue Perfusion and PO2 in Conscious Animals to Investigate Organ Failure

To watch the complete webinar click here