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(June 22, 2023) Webinar: iNSiGHT & PIXImus: A Tale of Two Systems

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(June 22, 2023) Webinar: iNSiGHT & PIXImus: A Tale of Two Systems

Overview

Dual X-ray absorptiometry allows measurements of fat mass, lean mass, bone mineral density, and bone mineral content in a live animal. The short exposure time allows for measurements to be performed on mice using relatively little anesthesia (e.g., isoflurane). Therefore, DXA is a powerful tool to frequently measure multiple parameters throughout the life of an animal. Over the years, multiple DXA systems were implemented with different strengths and weaknesses. Here, we are comparing the PIXImus and the new iNSiGHT models for measurements in adult mice. Overall, the two software systems are similar, and analyses show comparable results between the PIXImus and the iNSiGHT. However, there are significant differences when considering logistics. The PIXImus system is significantly smaller and lighter, making it more movable and space-friendly than the iNSiGHT. The PIXImus measurement time is higher making the measurements of large cohorts of mice markedly longer. Unlike the iNSiGHT system, the PIXImus machine is not fully shielded; therefore, restricting the area in which it can be placed (e.g., radiation rooms). In conclusion, the selection of a system would depend on the need of scientists and the available space for the machine.

Date

Jun 22 2023
11:00 am - 12:00 pm

Speaker

  • David Maridas PhD
    David Maridas PhD Research Fellow in Developmental Biology, Harvard School of Medicine
    David Maridas PhD
    David Maridas PhD
    Research Fellow in Developmental Biology, Harvard School of Medicine

    David is currently an Instructor in Developmental Biology at the Harvard School of Dental Medicine. During his graduate training, he acquired extensive training in skeletal biology using mouse models. David studied how different physiological and exogenous factors such as Insulin Growth Factor Binding Protein 4 (IGFBP4) and parathyroid hormone (PTH) can affect bone mass, architecture and function. Later, he designed experiments with nutritional manipulations including obesogenic and anorexic diets for mice and investigated their effects on metabolism and skeletal biology. Currently, his postdoctoral research is focusing on TGF-b superfamily ligands and receptors and demonstrated that their manipulation could affect peak bone mass acquisition and age-related bone loss in mice.

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