The USphere series of microbubble contrast agents have been specifically designed for use in a variety of species used in preclinical research, and may be used with ultrasound systems operating at 1 to 40MHz. Microbubble contrast agents can be used to visualize perfusion in a wide variety of imaging targets including tumors, abdominal organs, etc. and may be relevant from basic research studies to translational drug development projects.

Trust Bio-sonics Inc., a pharmaceutical company based in Taiwan, was founded in 2013 with a focus on developing products related to early diagnosis and treatment of cancers. The research and development team, comprised of experts from top research institutions from around the world, work to integrate their experience with microbubbles and liposomal formulations to develop new treatment solutions. Trust Bio-sonics offers both clinical and preclinical microbubble solutions

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The USphere series of microbubble contrast agents have been specifically designed for use in a variety of species used in preclinical research, anywhere from mice, rats, rabbits and even pigs; and have been tested and shown to work effectively at frequencies from 1 to 40MHz. Specifically, these agents have been tested on our Prospect T1 system and been found to perform well with both linear and harmonic contrast imaging modes.

There are a variety of bubble configurations available, those to study perfusion alone, others to aid in biomarker detection, and again others to perform ultrasound-mediated drug or gene delivery, as well as more advanced theranostic applications.

Trust Bio-sonics has identified the key factors which control the particle size of the microbubble. By precisely adjusting the lipid formulation, the USphere agents have been designed to have the smallest size distribution (1.1-1.4µm) compared to others on the market today. The USphere agents have a phospholipid shell filled with perfluoropropane (C3F8) or optional perfluorobutane (C4F10). Additionally, the microbubble concentration has been optimized for small animal imaging (~2.5×1010 bubbles/ml), ensuring low volume injections provide adequate contrast signal in without overwhelming their circulatory system.

Microbubble contrast agents can be used to visualize all levels of the vasculature, providing detailed perfusion information which may be relevant in tumor and abdominal organ imaging, as well as in drug development studies, just to name a few.

Product Line Product Name Description
USphereTM Prime

Prime (C3F8)

USphere Prime agents are plain microbubbles providing resilience for high-quality perfusion imaging.

This agent contains perfluoropropane (C3F8) and a phospholipid shell.

USphereTM Prime

Prime (C4F10)

USphere Prime (C4F10) agents are very similar to the above Prime agent, however, they contain perfluorobutane (C4F10) and a phospholipid shell.

This plain microbubble may be selected to perform marginally better with specific ultrasound systems, based on the frequency of sound and the acoustic energy in Contras Mode.

The USphere agents can be stored at 2-8oC for up to 6 months.

Once activated, using the UltraMixTM agitator (one-time purchase), the agent can be used for up to 3 days with greater than 90% performance, when stored between 2-8oC when not in use.

Product Line Product Name Description
USphereTM Tracer Tracer FD-Red USphere Tracer FD agents are plain microbubbles which have been modified to have a fluorescent dye inside to be used in studies which combine ultrasound and optical imaging techniques.

The fluorescent dye could be used with in vivo optical imaging techniques to model the biodistribution of a drug delivery/release to study pharmacokinetics.

The Tracer FD-Red agent utilizes DiI, with excitation at 549nm and emission at 565nm.

USphereTM Tracer Tracer FD-Green Tracer FD-Green agents utilize DiO with excitation at 484nm and emission at 501nm.

The USphere agents can be stored at 2-8oC for up to 6 months.

Once activated, using the UltraMixTM agitator (one-time purchase), the agent can be used for up to 3 days with greater than 90% performance, when stored between 2-8oC when not in use.

Product Line Product Name Description
USphereTM Labeler Labeler LB (Biotin) USphere Labeler LB agents have been functionalized with specific molecules to allow researchers to conjugate targeting moieties or antibodies to the microbubble.

Different targeting moieties can be used for biomarker detection in a variety of disease models including angiogenesis (VEGFR2), inflammation (P-selectin and integrins), ischemia and thrombosis, etc.

The Labeler LB (Biotin) agent has been functionalized with a biotin molecule on the phospholipid shell.

USphereTM Labeler Labeler LA (Biotin and Avidin) The Labeler LA (Biotin and Avidin) agent has been functionalized with biotin-avidin molecule on the phospholipid shell.
USphereTM Labeler Labeler FLA (LA with Fluorescence) The Labeler FLA (Biotin and Avidin) agent has been functionalized with a biotin-avidin molecule on the phospholipid shell; additionally, FITC is linked to the avidin molecule to confirm the targeted site of the microbubbles by other techniques.
USphereTM Labeler Labeler LS (Biotin and Streptavidin) The Labeler LS (Biotin and Streptavidin) agent has been functionalized with a biotin-streptavidin molecule on the phospholipid shell.

The USphere agents can be stored at 2-8oC for up to 6 months.

Once activated, using the UltraMixTM agitator (one-time purchase), the agent can be used for up to 3 days with greater than 90% performance, when stored between 2-8oC when not in use.

Product Line Product Name Description
USphereTM Trans+ Trans+ TP USphere Trans+ TP agents have been functionalized with a positively charged surface. These microbubbles can easily yield a high payload of DNA or RNA making gene transfection more targeted, effective, and simpler than traditional gene transfection techniques. Note – Sonoporation is needed in combination with these microbubbles to facilitate gene transfection at a targeted location.

The USphere agents can be stored at 2-8oC for up to 6 months.

Once activated, using the UltraMixTM agitator (one-time purchase), the agent can be used for up to 3 days with greater than 90% performance, when stored between 2-8oC when not in use.

USphere Prime Agents

Prime Agents are plain microbubbles providing resilience for high quality perfusion imaging. These agents contain perfluoropropane (C3F8) or perfluorbutane (C4F10) and a phospholipid shell


System

Images
  High-Frequency Ultrasound Imaging Systems

  Prospect T1 System

Manufactured by S-Sharp, distributed by Scintica

  • Image was taken on an orthotopic liver tumor in a mouse, using the USphere Prime agent
  • Please note that harmonic, non-linear, contrast imaging is now possible on the Prospect T1 system. The images below were taken with the linear contrast mode which was previously available.
   Ultrasound Images

Vevo 2100 system, manufactured by VisualSonics

  • Image was taken on normal mouse kidney and liver, using the USphere Prime agent
     Clinical Ultrasound Imaging Systems

     Toshiba system

  • Image was taken on a pig, showing the ablation site in the liver, using the USphere Prime agent
      Ultrasound Images

        Zonare system 

  • Image was taken on a rabbit, showing kidney perfusion, using the USphere Prime agent


USphere Tracer Agents

Tracer Agents are plain microbubbles which have been modified to have a fluorescent dye inside to be used in studies which combine ultrasound and optical imaging techniques. The fluorescent dye could be used with in vivo optical imaging techniques to model the biodistribution of a drug delivery/release to study pharmacokinetics. Available options include red, green and orange dyes.



USphere Labeler Agents

 Labeler agents have been functionalized with specific molecules to allow researchers to conjugate targeting moieties or antibodies to the microbubble.Different targeting moieties can be used for biomarker detection in a variety of disease models including angiogenesis (VEGFR2), inflammation (P-selectin and integrins), ischemia and thrombosis, etc. These Labeler agents may also contain a fluorescent dye to aid in multi-modal imaging.



USphere Trans+ Agents

Trans+ Agents have been functionalized with a positively charged surface. These microbubbles can easily yield a high payload of DNA or RNA making gene transfection more targeted, effective, and simpler than traditional gene transfection techniques.