There have been a number of published reports recently suggesting a substantial amount of cancer related studies published in high-impact scientific journals could not be reproduced outside of their originator laboratories. These discoveries are remarkably problematic as many of the drugs screened are being developed past the preclinical stage, into human testing
This free webinar hosted by Scintica Instrumentation provided researchers with an overview on the effects of oxygen during in vitro cell culturing. Further, the discussion focused on how to accurately determine and regulate the O2 levels during experiments. Last,we took researchers through our extensive list of hypoxia/physoxia options and guide them down
Cancer micro-environments are essential in understanding cancer malignancy and can provide accurate insights into how therapies targeting tumors may respond. Looking into these micro-environments further, Scintica Instrumentation’s cellular oxygen expert, Justin Croft, teamed up with custom media specialist, Annie Cunningham, of InVitria to publish the article “How modern hypoxia chambers and chemically defined media are changing the
The lecture “Elucidation of oxygen sensing pathways in human and animal cells; implications for Physiology” by esteemed professor Dr. Peter Ratcliffe of Oxford University delves into the topic of physoxia head on. Dr. Ratcliffe provides relevant and interesting data from across his career’s work in solving mechanisms by which cells sense and signal hypoxia. The
What oxygen levels are you using in your cellular research? Are your cells under hyperoxia, normoxia, hypoxia, or are you unsure? This free webinar hosted by Scintica Instrumentation provided our audience with an overview on the effects of oxygen during in vitro cell culturing. Further, the discussion focused on how
Since the first CO2 incubators were made commercially available in the late 1960’s they have become a staple for researchers to grow and maintain cells in vitro. These chambers have been highly effective for this task; however, they do not take into account one significant factor - oxygen. Currently, there is a push from
In the last year, I’ve spoken to countless hypoxia researchers in person, at conferences, and over the phone. When I reach out to these people the one question I always ask is “in what units does your current chamber report measured oxygen content?” to which I almost always get a resounding answer of percentage
The review article “Frequently asked questions in hypoxia research” by Wenger et al. covers several issues every researcher performing In vivo or In vitro hypoxia-based research should think about before performing their studies. This article is meant to help those in the early stages of attempting hypoxic studies. It should also be useful to
