The Newton 7.0 is an innovative optical bioluminescence, fluorescence, and 3D tomographic imaging system designed with the user in mind. It is ideal for in vivo, ex vivo and in vitro imaging applications, allowing for simultaneous imaging of multiple animals or samples at a time. It’s advanced features and software are easy to navigate and optimized for a multi-user interface. Furthermore, the intuitive workflow and advanced system sensitivity facilitates time-saving signal acquisition for longitudinal studies.
VILBER is a leading life science company developing and manufacturing fluorescence, chemiluminescence and bioluminescence imaging systems for applications ranging from small animal to cell biology research. Founded in 1954, Vilber is a leader in the molecular imaging sector, equipping more than 20,000 laboratories worldwide. An estimated 60,000 people use their products every day in over 100 countries.
The Newton 7.0 is a highly sensitive optical imaging system dedicated to pre-clinical imaging of small animals in vivo, and may also be used on a variety of in vitro and ex vivo samples. It combines the best optics and animal handling features for optimum scientific images and results. The Newton 7.0 systems are capable of bioluminescence, fluorescence as well as 3D tomographic imaging. The system is:
Does not require any radiation to acquire images
Is non-invasive, allowing for longitudinal studies
Allows for up to 5 mice or 3 rats to be imaged simultaneously
Powerful Fluorescence Excitation
The Newton 7.0 offers 8 excitation channels in the visible RGB and near infrared spectrums. The very tight LED spectrum is additionally constrained with a very narrow excitation filter; these excitation sources are categorized as a Laser Class II due to their intense power. Movement of the excitation source over the entire FOV ensures consistent and reproducible results over the course of a longitudinal study
Full Spectrum Tunability
8 excitation channels and 8 emission filters are available to cover the complete spectrum from Blue to infra-red.
Narrow bandpass filters are used for both excitation and emission to reduce cross talk between dies, allowing for up to 3 dyes to be imaged simultaneously
Macro-imaging to large throughput studies
Vilber’s intelligent darkroom architecture allows for fully automated movement of the camera (Z-axis) and animal pad (X/Y axis) to move through both the macro imaging FOV (6x6cm) to the full FOV (20x20cm) for imaging up to 5 mice
Spectral unmixing is possible for both bioluminescence and fluorescence imaging when different luciferase enzymes or fluorescent dies are used.
The includes algorithms to remove crosstalk between the different signals, allowing for each channel to contain signal from only one reporter
3D Optical Tomography
An integrated 3D tomography module allows both bioluminescence and fluorescence signals to be reconstructed in 3D and overlaid within a topographical model of the imaging subject.
For better understanding of anatomical and deeper tissue structures, the digital organ library allows for superimposition of the mouse organs and bones onto the topographical model
State-or-the-art camera technology:
Scientific grade 16-bit CCD
-90oC delta cooling
10 megapixel image resolution
4.8 Optical Density
The advanced camera and optics provide increased sensitivity to either bioluminescence or fluorescence signals, with a very low signal to noise ratio. The high optical density allows for samples with both very low and high signals to be imaged without saturation, allowing for quantifiable results
Images acquired at different time points can be arranged to form a longitudinal image sequence. For example, a time series could be constructed from images acquired on different days following an experimental treatment.
The software then compares the image data throughout the experimental treatment.
Day 1 Day 5 Day 10 Day 15 Day 20 Day 25 Day 30 Day 35
Using the Vilber imaging system, researchers investigated the biodistribution of doxorubicin hydrochloride-loaded nanogels in rats (Sprague–Dawley, 220–250 g), click here for the link to the article abstract. The fluorescent signal of DOX was detected and monitored over eight hours, seen below.
The major organs were dissected 10 h after oral administration and observed ex vivo.
Signal quantification demonstrated that organs harvested from rats treated with doxorubicin hydrochloride-loaded nanogels group exhibited significantly higher retention of doxorubicin hydrochloride compared to rats treated with doxorubicin hydrochloride alone.
In vivo fluorescence imaging of FITC-BSA nanoparticle in the Turbot fish.
After 36 h, the heart and liver were dissected and visualized nanoparticle retention was identified.
Distribution of FITC-BSA in turbot fish at different time points In vivo fluorescence imaging of FITC-BSA distribution in organs at 36 h
Bioluminescent enterotoxic E. coli (ETEC) is tracked through the mouse intestine, demonstrating the colonization dynamics across the GI tract. Click here for the link to the article abstract
Streptomycin-treated BALB/c mice were inoculated with E. coli bacteria via gavage with pRMkluc-tagged ETEC and pRMkluc-tagged E. coli K-12. Luciferin was administered intraperitoneally prior to imaging.
After inoculation, bioluminescence was localized to the small intestine. 48 hours post-inoculation, the bioluminescent signals indicated bacterial passage through the mouse intestine. Bioluminescent signals were detected in the mouse intestine up to 120 h post-inoculation.
After 120 h of E. coli infection, mouse gastrointestinal tracts were extracted to perform ex vivo imaging. Intestinal tract dissection included the esophagus to rectum.
ETEC were localized in the proximal mouse ileum approximately 6 cm from the cecum, whereas the E. coli K-12 ol signals were identified in the cecum and in the proximal colon.
BALB/c mice were intraperitoneally inoculated with E. coli K-12 tagged with pRMkluc or E. coli K-12 tagged with pBR322 (incubated with luciferin). Click here for a link to the article abstract.
After 1 h of infection, bioluminescent signal emission from the animals was captured.
Mice infected with E. coli K-12 harboring pBR322 did not exhibit bioluminescent emission (Fig.B). However, mice infected with E. coli harboring pRMkluc emitted bioluminescent signals detected in the mouse inoculation zone (Fig.A).