The Newton 7.0 is an innovative optical bioluminescence, fluorescence, and 3D tomographic imaging system designed with the user in mind. It is ideal for in vivo, ex vivo and in vitro imaging applications, allowing for simultaneous imaging of multiple animals or samples at a time. It’s advanced features and software are easy to navigate and optimized for a multi-user interface. Furthermore, the intuitive workflow and advanced system sensitivity facilitates time-saving signal acquisition for longitudinal studies.
VILBER is a leading life science company developing and manufacturing fluorescence, chemiluminescence and bioluminescence imaging systems for applications ranging from small animal to cell biology research. Founded in 1954, Vilber is a leader in the molecular imaging sector, equipping more than 20,000 laboratories worldwide. An estimated 60,000 people use their products every day in over 100 countries.
The Newton 7.0 is a highly sensitive optical imaging system dedicated to pre-clinical imaging of small animals in vivo, and may also be used on a variety of in vitro and ex vivo samples. It combines the best optics and animal handling features for optimum scientific images and results. The Newton 7.0 systems are capable of bioluminescence, fluorescence as well as 3D tomographic imaging. The system is:
- User-friendly
- Does not require any radiation to acquire images
- Is non-invasive, allowing for longitudinal studies
- Allows for up to 5 mice or 3 rats to be imaged simultaneously
| Feature | Benefit |
|---|---|
| Powerful Fluorescence Excitation | The Newton 7.0 offers 8 excitation channels in the visible RGB and near infrared spectrums. The very tight LED spectrum is additionally constrained with a very narrow excitation filter; these excitation sources are categorized as a Laser Class II due to their intense power. Movement of the excitation source over the entire FOV ensures consistent and reproducible results over the course of a longitudinal study |
| Full Spectrum Tunability | 8 excitation channels and 8 emission filters are available to cover the complete spectrum from Blue to infra-red. Narrow bandpass filters are used for both excitation and emission to reduce cross talk between dies, allowing for up to 3 dyes to be imaged simultaneously |
| Macro-imaging to large throughput studies | Vilber’s intelligent darkroom architecture allows for fully automated movement of the camera (Z-axis) and animal pad (X/Y axis) to move through both the macro imaging FOV (6x6cm) to the full FOV (20x20cm) for imaging up to 5 mice |
| Spectral Unmixing | Spectral unmixing is possible for both bioluminescence and fluorescence imaging when different luciferase enzymes or fluorescent dies are used. The includes algorithms to remove crosstalk between the different signals, allowing for each channel to contain signal from only one reporter |
| 3D Optical Tomography | An integrated 3D tomography module allows both bioluminescence and fluorescence signals to be reconstructed in 3D and overlaid within a topographical model of the imaging subject. For better understanding of anatomical and deeper tissue structures, the digital organ library allows for superimposition of the mouse organs and bones onto the topographical model |
State-or-the-art camera technology:
| The advanced camera and optics provide increased sensitivity to either bioluminescence or fluorescence signals, with a very low signal to noise ratio. The high optical density allows for samples with both very low and high signals to be imaged without saturation, allowing for quantifiable results |
Fluorescence imaging can be used to detect fluorescent reporter genes or dyes in vivo using Vilber’s dynamic range of excitation filters.
Subcutaneous tumor expressing mCherry
The below dyes can be used with the Newton 7.0 systems:
Bioluminescence imaging can be used to detect luciferase expressing or secreting molecules in the tissue of interest
Subcutaneous tumor expressing firefly luciferase
Multispectral in vivo imaging is possible by using different luciferase enzyme/substrate pairs or by using different fluorescent dyes.
Signals can be overlaid within the same image, up to 3 reporters can be imaged simultaneously.
Images acquired at different time points can be arranged to form a longitudinal image sequence. For example, a time series could be constructed from images acquired on different days following an experimental treatment.
The software then compares the image data throughout the experimental treatment.
Day 1 Day 5 Day 10 Day 15 Day 20 Day 25 Day 30 Day 35
Oncology
“Optical imaging can be used to noninvasively monitor the progression and spread of cancer throughout the body in preclinical animal models”
Immunology
“Monitoring various populations of immune cells can contribute significantly to the understanding of their physiology and the development of new therapeutic strategies’
Infectious disease
”Optical imaging can be used to noninvasively visualize a site of infection as well as the efficacy of a treatment in the context of living subjects”
Neurology
”Optical imaging can be used to monitor the progression of various neurodegenerative diseases as well as to test novel targeted therapeutics within the brain and spinal cord”
Biodistribution studies
”The ability to image the whole subject, gives optical imaging a unique advantage in preclinical biodistribution studies, such that one image can provide measurements for multiple organs throughout the body”
| F E A T U R E S | NEWTON 7.0 BT100 | NEWTON 7.0 BT500 | NEWTON 7.0 FT100 | NEWTON 7.0 FT500 |
|---|---|---|---|---|
 |  | | | |
| M O D A L I T Y | In vivo / in vitro Optical imaging platform Bioluminescence detection 3D Optical Tomography _ | In vivo / in vitro Optical imaging platform Bioluminescence detection Fluorescence detection D Optical Tomography | ||
| O P T I C S | ||||
C a m e r a | 16-bit Scientific Grade CCD Camera Grade 0 / 400-900nm / 4.8 OD Cooling: -90°C Delta
| 16-bit Scientific Grade CCD Camera Grade 0 / 400-900nm / 4.8 OD Cooling: -90°C Delta
| 16-bit Scientific Grade CCD Camera Grade 0 / 400-900nm / 4.8 OD Cooling: -90°C Delta
| 16-bit Scientific Grade CCD Camera Grade 0 / 400-900nm / 4.8 OD Cooling: -90°C Delta
|
L e n s | Proprietary V.070 – Fixed Focal Length Motorized lens Aperture: f/0.7 | Proprietary V.070 – Fixed Focal Length Motorized lens Aperture: f/0.7 | Proprietary V.070 – Fixed Focal Length Motorized lens Aperture: f/0.7 | Proprietary V.070 – Fixed Focal Length Motorized lens Aperture: f/0.74 |
R e s o l u t i o n | Resolution: 2160×2160 Monochrome & Color imaging | Resolution: 2160×2160 Monochrome & Color imaging | Resolution: 2160×2160 Monochrome & Color imaging | Resolution: 2160×2160 Monochrome & Color imaging |
F i e l d o f V i e w a | 1 animal capacity FOV Min: 12x12cm FOV Max: 12x12cm 3D imaging mode via 3D-IR cameras | 5 animals capacity FOV Min: 6x6cm FOV Max: 20x20cm Macro imaging mode 3D imaging mode via 3D-IR cameras | 1 animal capacity FOV Min: 6x6cm FOV Max: 20x20cm Macro imaging mode 3D imaging mode via 3D-IR cameras | 5 animals capacity FOV Min: 6x6cm FOV Max: 20x20cm Macro imaging mode 3D imaging mode via 3D-IR cameras |
E X C I T A T I O N | ||||
W h i t e – L i g h t | Dual EPI-White light LED panels | Dual EPI-White light LED panels | ||
F l u o r e s c e n c e | Upgradeable to Fluorescence | 8 Fluorescent channels included 420 / 480 / 520 / 580 / 640 / 680 / 740 / 780nm | ||
E M I S S I O N | ||||
F i l t e r W h e e l | 10-position Motorized filter wheel | 10-position Motorized filter wheel | ||
E m m i s s i o n | 4 Narrow Band-pass filters included for the BLI Tomography 500 / 550 / 600 / 650nm | 8 Narrow Band-pass filters included for the BLI Tomography 500 / 550 / 600 / 650nm | ||
D A R K R O O M | ||||
| M o t o r i z a t i o n | Fixed Camera Fixed Animal Stage | Z-Axis motorized camera X/Y-Axis motorized Animal Stage | Fixed Camera Fixed Animal Stage | Z-Axis motorized camera X/Y-Axis motorized Animal Stage |
| A N I M A L H A N D L I N G | Heated Mouse Bed (+37°C) included Animal breathers included | |||