Easily detect fluorescent molecules and reporters at the picogram level in the tissue of interest using Vilber’s dynamic range of excitation emission wavelengths.

For deeper tissue penetration and reduction of autofluorescence background, infrared (IF) and near-infrared (NIR) molecules can also be imaged with Vilber.

The below dyes can be used with the Newton 7.0 systems:

Bioluminescence imaging can be used to detect luciferase-tagged molecules at the femtogram level.

Multispectral in vivo imaging is possible by using different luciferase enzyme/substrate pairs or by using different fluorescent dyes.

Signals can be overlaid within the same image, up to 3 reporters can be imaged simultaneously.

Images acquired at different time points can be arranged to form a longitudinal image sequence. For example, a time series could be constructed from images acquired on different days following an experimental treatment.

The software then compares the image data throughout the experimental treatment.

 
           Day 1                               Day 5                             Day 10                              Day 15                           Day 20                          Day 25                             Day 30                            Day 35

Inflammatory responses can be assessed using non-invasive fluorescence biomarker imaging in a preclinical model of rheumatoid arthritis.

Using the Vilber imaging system, researchers investigated the biodistribution of doxorubicin hydrochloride-loaded nanogels in rats (Sprague–Dawley, 220–250 g), click here for the link to the article abstract. The fluorescent signal of DOX was detected and monitored over eight hours, seen below.

The major organs were dissected 10 h after oral administration and observed ex vivo.

Signal quantification demonstrated that organs harvested from rats treated with doxorubicin hydrochloride-loaded nanogels group exhibited significantly higher retention of doxorubicin hydrochloride compared to rats treated with doxorubicin hydrochloride alone.

In vivo fluorescence imaging of FITC-BSA nanoparticle in the Turbot fish.

After 36 h, the heart and liver were dissected and visualized nanoparticle retention was identified.

                           
Distribution of FITC-BSA in turbot fish at different time points                                                                          In vivo fluorescence imaging of FITC-BSA distribution in organs at 36 h

Bioluminescent enterotoxic E. coli (ETEC) is tracked through the mouse intestine, demonstrating the colonization dynamics across the GI tract. Click here for the link to the article abstract

Streptomycin-treated BALB/c mice were inoculated with E. coli bacteria via gavage with pRMkluc-tagged ETEC and pRMkluc-tagged E. coli K-12.  Luciferin was administered intraperitoneally prior to imaging.

After inoculation, bioluminescence was localized to the small intestine. 48 hours post-inoculation, the bioluminescent signals indicated bacterial passage through the mouse intestine. Bioluminescent signals were detected in the mouse intestine up to 120 h post-inoculation.

After 120 h of E. coli infection, mouse gastrointestinal tracts were extracted to perform ex vivo imaging. Intestinal tract dissection included the esophagus to rectum.

ETEC were localized in the proximal mouse ileum approximately 6 cm from the cecum, whereas the E. coli K-12  ol signals were identified in the cecum and in the proximal colon.

BALB/c mice were intraperitoneally inoculated with E. coli K-12 tagged with pRMkluc or E. coli K-12 tagged with pBR322 (incubated with luciferin). Click here for a link to the article abstract.

After 1 h of infection, bioluminescent signal emission from the animals was captured.

Mice infected with E. coli K-12 harboring pBR322 did not exhibit bioluminescent emission (Fig.B). However, mice infected with E. coli harboring pRMkluc emitted bioluminescent signals detected in the mouse inoculation zone (Fig.A).

in vivo bioluminescence emission of E. coli K-12

Screen localization dynamics of fluorescently-tagged drugs. Investigators injected a Cy5.5-tagged drug and tracked it’s spread systemically in the mouse.

Tumor progression can be monitored after establishment of orthotopic tumors in mice. The mouse brain was injected with 10,000 (fig.a ) and 50,000 (fig.b ) cancer cells expressing luciferase.

After a 6 weeks, tumors were formed, where signal was dependent on tumor cell injection quantity.

Fig a: 10 000 tumoral cells injected in the brain                                                                                                      Fig a: 50 000 tumoral cells injected in the brain