RWD RFLSI III Laser Speckle Perfusion Imager

RFLSI III is based on the LSCI (Laser Speckle Contrast Imaging) technology design. With the advantages of its non-contact, high time resolution, high spatial resolution, and full-field rapid imaging, it provides a real-time dynamic blood flow monitoring and recording method for research in life sciences. The RFLSI III provides the basis for understanding tissue and organ pathological or physiological indexes vital data. Contrast agent is not needed, time resolution can be in milliseconds, spatial resolution can be in microns. RWD adopted a new all-in-one design to improve the operability for the user.

Benefits:

  • Continuous Imaging, No Need to Scan

  • High Time & Spatial Resolution and High Contrast

  • Multi Ouput: Video, Image, Blood Flow Data, Diameter Analysis

  • Non Contact, No Contrast Agent

  • No Damage to the Animal

  • All in One Design

Performance Advantages

  • Analyze vascular perfusion volume, tube diameter, tube angle, and other data online and offline to meet hemodynamic research.
  • Multiple data acquisition methods including continuous acquisition, specified interval acquisition, specified frame number acquisition, etc.
  • An indicating laser plus autofocus function makes it easy to use the
    instrument.
  • High-definition camera (full-frame 2048 x 2048 resolution) helps you see the blood vessel endings and opens the world of blood vessel microcirculation images.
  • The high-speed camera (up to a maximum frame rate of 140 frames per second) allows recording of changes every second and recording of more details of blood vessel changes post-intervention.
  • Package-type support solutions and timely after-sales service will help you
    be one step ahead in your scientific research

Applications

  • Vascular regeneration in the repair of auricle damage in nude mice;
  • Functional response of the cerebral cortex during cerebral ischemia and reperfusion in rats and mice;
  • Functional response of blood flow in the somatosensory region of the cerebral cortex in rats and mice;
  • Light and electricity functional responses to blood flow in somatosensory regions of mouse cortex induced by external stimuli;
  • Brain function and brain injury research;
  • Cerebral blood flow imaging related to cerebral cortex, physiology, and pathology;
  • Circulation and metabolism under various physiological and pathological conditions;
  • Animal models of pathology in vivo imaging of cerebral cortex blood channels (such as MCAO);
  • Study of intestinal mucosal blood vessels;
  • Study of lower limb ischemia and vascular survival in rats and mice;
  • Study of cortical diffusion inhibition.

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Technical Parameters

  • The range of the blood flow monitoring is from zero to infinity. The spatial resolution of the blood flow imaging is 3.3μm/pixel, and the maximum is 2μm/pixel
  • Laser type: laser diode, wavelengths 785nm, monitoring camera power 90mW
  • Highly coherently stable laser light source and low noise 12bit fast camera ensure the stability of excellent flow-rate measurement
  • Monitoring distance: 110mm, directly read the magnification, no autofocus measurement distance calibration
  • In the recording process, choose any number of blood vessels, real-time display diameter changes and angle
  • The effective pixels in the unit area are no less than 1800000 pixels /cm2; the monitoring pixel is 656*494 pixels
  • With two modes of continuous recording and interval recording
  • The image monitoring area is 14.4*10.9-2.15*1.62mm, and the imaging speed is not less than 60 fps in full amplitude state
  • ROI area and vessel diameter measurements are be added arbitrarily during the recording process or off-line analysis to support any shape and number of ROI choices
  • Optical magnification: conventional 0.67-4.5 times, 0.071NA, optional 0.5 times, 2 times auxiliary objective lens; increase the auxiliary objective lens, the monitoring area and blood flow imaging spatial resolution will be adjusted accordingly
  • The monitoring records can be exported to AVI format video files, including curves, blood flow, experimental process records,the output video can be adjusted as required

Example Images

Tree shrew (nonhuman primate) cerebral cortical blood flow imaging

(a) Normal cerebral cortical blood flow distribution of tree shrew; (b) The distribution of cerebral cortical blood flow when start to over anesthesia; (c) The distribution of dying cerebral cortical blood flow when severe overdose of anesthesia.

Cerebral cortical blood flow distribution of the rat mini-stroke model

(a) Normal cerebral cortical blood flow distrbution; (b) Cortical blood flow distribution after established the mini-stroke model;
(c) Cortical blood flow distribution after removal of the mini-stroke model for 24h

Photochemical induction of mouse cerebral cortex ischemia model

Photochemical induction of mouse cerebral cortex ischemia model