RWD RFLSI Pro Laser Speckle Perfusion Imager

A new RFLSI Pro Laser Speckle Perfusion Imager is based on the latest LSCI technology (Laser Speckle Contrast Imaging/LSCI, Laser Speckle Ratio Analysis). With the advantages of the unique non-contact, high time and spatial resolution, rapid imaging, it provides a new recording way for the life sciences basic research and clinical medical, which can achieve real-time dynamic blood flow monitoring and video imaging, it is the vital basis for understanding the organization, organ pathology and physiological indicators.. Contrast agent is not needed, time resolution can be in milliseconds, spatial resolution can be in microns. It also achieves the requirements of real-time observation of microvascular blood flow distribution and relative changes in values. RWD odopts a new all in one design to improve the operability for the user.

Benefits:

  • Continuous Imaging, No Need to Scan

  • High Time & Spatial Resolution and High Contrast

  • Multi Ouput: Video, Image, Blood Flow Data, Diameter Analysis

  • Non Contact, No Contrast Agent

  • No Damage to the Animal

  • All in One Design

Applications:

  • CBF

  • MCAO Model

  • Gastrointestinal Blood Flow

  • Lower Limb Ischemia

  • Angiogenesis

  • Burn Assessment

  • Skin Patch Test

  • Cerebral Cortex Diffusion Inhibition 

Performance Advantages

  • Real-time monitoring of two-dimensional blood flow images in microns. Use corrected static tissue structure interference and multiple scattering effects algorithm to quantitatively analyze blood flow changes and improve the accuracy of blood flow monitoring in vessels of different diameter
  • With the function of real-time online and off-line analysis of mean flow-rate and the vascular diameter in ROI (Region of Interest), and off-line analysis of mean and standard deviation of ROI (Region of Interest), supporting the selection, copy, and deletion of ROI for any shape and quantity, and the ROI position and size\ can be adjusted with the mouse by free dragging and dropping
  • Maintain a high imaging speed of not less than 60fps under Full-Frame state (the camera’s area array is all used)
  • The positioning grid can be adjusted arbitrarily to meet the image positioning of different groups of experiments
  • The distance scale and real-time ROI area are added to facilitate the acquisition of relevant information
  • Real-time focal length adjustment, read the magnification directly, no calculations required
  • Select multiple vessels, online/off-line analysis of vascular diameter and its changes to meet the requirements of hemodynamics research
  • Multiple data storage format: Original blood flow-rate data /Standard images in various formats (jpg, bmp, png) /.avi format video / etc.
  • Multiple blood flow image acquisition methods: flexible continuous acquisition, designated time interval acquisition, designated frame number acquisition, etc.
  • Highly coherently stable laser light source and low noise 12bit fast camera ensure the stability of excellent flow-rate measurement.
  • All-inclusive supporting solutions and after-sales service
  • The function of saving and load of ROI files has been added to meet the requirement of synchronous ROI analysis between different groups of experiments

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Technical Parameters

Example Images

  • The range of the blood flow monitoring is from zero to infinity. The spatial resolution of the blood flow imaging is 3.3μm/pixel, and the maximum is 2μm/pixel
  • Laser type: laser diode, wavelengths 785nm, monitoring camera power 90mW
  • Highly coherently stable laser light source and low noise 12bit fast camera ensure the stability of excellent flow-rate measurement
  • Monitoring distance: 110mm, directly read the magnification, no autofocus measurement distance calibration
  • In the recording process, choose any number of blood vessels, real-time display diameter changes and angle
  • The effective pixels in the unit area are no less than 1800000 pixels /cm2; the monitoring pixel is 656*494 pixels
  • With two modes of continuous recording and interval recording
  • The image monitoring area is 14.4*10.9-2.15*1.62mm, and the imaging speed is not less than 60 fps in full amplitude state
  • ROI area and vessel diameter measurements are be added arbitrarily during the recording process or off-line analysis to support any shape and number of ROI choices
  • Optical magnification: conventional 0.67-4.5 times, 0.071NA, optional 0.5 times, 2 times auxiliary objective lens; increase the auxiliary objective lens, the monitoring area and blood flow imaging spatial resolution will be adjusted accordingly
  • The monitoring records can be exported to AVI format video files, including curves, blood flow, experimental process records,the output video can be adjusted as required

Tree shrew (nonhuman primate) cerebral cortical blood flow imaging

(a) Normal cerebral cortical blood flow distribution of tree shrew; (b) The distribution of cerebral cortical blood flow when start to over anesthesia; (c) The distribution of dying cerebral cortical blood flow when severe overdose of anesthesia.

Cerebral cortical blood flow distribution of the rat mini-stroke model

(a) Normal cerebral cortical blood flow distrbution; (b) Cortical blood flow distribution after established the mini-stroke model;
(c) Cortical blood flow distribution after removal of the mini-stroke model for 24h

Photochemical induction of mouse cerebral cortex ischemia model

Photochemical induction of mouse cerebral cortex ischemia model