Bioprinting allows for the fabrication of scaffolds, cells, tissues, and organs with high accuracy, reproducibility and specificity. These technologies utilize cells or cell factors as a “bioink” to fabricate prospective tissue structures. Recently, immense progress has been made in printing various types of tissue, including vasculature, heart, bone, cartilage, skin and liver, but many conventional tissue engineering and 3D bioprinting techniques are still limited to coarse resolution of 300-500 μm.
The Next Generation Bioprinting (NGB) platform from Poietis has been developed to overcome current tissue manufacturing shortfalls and solve critical limitations of existing 3D bioprinting technologies thanks to single-cell resolution and learning-based methods. This platform integrates automation and robotics, and numerous online sensors – including cell microscopy – and Artificial Intelligence processing. In addition, it integrates all bioprinting techniques (laser-assisted bioprinting, bioextrusion and micro-valve bioprinting), a world’s first in the bioprinting market.
The NGB-R enables true versatility of bioprinting (from cells to spheroids) and offers the possibility of using a large number of biomaterials and hydrogels to achieve unparalleled technical capabilities including:
The NGB-R also includes an embedded microscope for in-line cell printing monitoring and relies on a complete software suite for managing bioprinting protocols, from biological CAD to data analysis of manufacturing.
|High resolution||The NGB-R is the first commercially available instrument to boast laser-assisted bioprinting, allowing users to deposit micro droplets (ranging in size from 50 μm to 300μm) of cell bioink with a precision of a few microns. With these capabilities all levels of resolution are possible, from aggregates to cellular spots and single cells.|
|>95% cell viability||Many bioprinting instruments rely on extrusion-based technology which can lead to cell injury, damage, and a low cell-viability ratio. The NGB-R utilizes laser-assisted bio-fabrication (LAB) technology, a nozzle-free technique with no damage-causing forces. As a result, cell viability reaches >95% and printed tissues become truly functional.|
|Unrivaled 10μm precision||In additive tissue manufacturing, the initial positioning of cells has a huge impact on the evolution of future tissues. While ordinary extrusion- based bioprinting techniques do not allow for precise positioning of printed cells within the hydrogel, NGB-R offers the unique possibility to accurately arrange cells exactly where and as desired.|
|All-In-One Biofabrication Cabinet||The NGB-R features a user friendly, all-in-one biofabrication cabinet including:|
- Fully integrated biosafety cabinet
- High-end 6-axis robotic arm
- Large 21” touch-screen interface
- Bio-extrusion & micro-valve heads
- Exclusive laser bioprinting head
- Built-in cellular-level microscope
|Integrated Poietis Image Analysis (PIA) Software*|
|Stunning 3D image visualization|
- PIA works hand-in-hand with the NGB-R’s integrated microscope to deliver sharp images of each individual tissue layer and can reconstruct an impressive 3D representation of the entire tissue.
In-line 2D & 3D cell patterns analysis
- PIA performs cell patterns analysis which includes automatic and accurate cell counting based on advanced machine learning algorithms. PIA then ensures all wells have received an equal number of cells.
- PIA also calculates valid droplets percentage according to your preferences, parameters and desired number of cells per drop.
|Viewprint*||Many in vivo applications require users to print living cells into confined, microscopic areas. Poeitis’ patented technology, ViewPrint, is integrated into the NGB-R and offers unparalleled precision where it matters the most.|
How Does Laser Assisted Bioprinting Work?
Poetis’ laser assisted bioprinting (LAB) technology overcomes many of the technical limitations of extrusion based bioprinting methods and makes it possible to print living cells and biomaterials with a cell-level resolution. It is therefore the bioprinting technology that offers the highest resolution. Further, LAB allows cells to be positioned in three dimensions with micrometric precision allowing users total control over cell density, distribution and patterns within the printed tissues.
Laser-assisted bioprinting usually consists of three parts: a pulsed laser source, a cartridge (composed of an ink film spread on a glass plate), and a receiving substrate. The focusing of a laser pulse on a cartridge results in the formation of an ink jet towards a receiving substrate on which cell microdroplets are collected. By controlling the physical conditions of the ejection (energy, viscosity, etc), the volume of the droplets is controlled precisely (~ picoliter). The cell patterns are obtained by rapid scanning of the cartridge by the laser, which results in the formation of 10,000 droplets per second.
There are many advantages to printing living cells with LAB starting with viability. LAB is a nozzle-based technology which eliminates shear stress from the bioprinting process resulting in a cell viability ratio greater than 95%. Further, LAB allows for increased cell patterning possibilities and advanced tissue modeling due to the unprecedented capacity to spatially organize cells in 3D. The NGB-R not only offers LAB technology but combines it with conventional micro-valve, and extrusion bioprinting capabilities, resulting in the most advanced all-in-one bioprinting instrument for research applications.