Φ-eyeTM

A highly sensitivity, benchtop, in vivo imaging system for preclinical optical studies

Features & Benefits

System Overview

The φ-eye is a highly sensitive in vivo optical imaging system, designed for bioluminescence (BLI), fluorescence (FLI) and Cherenkov luminescence imaging (CLI). This system is used for a wide range of applications, including oncology and pharmacokinetic studies. With it’s highly sensitive detector, small footprint, and field of view suitable for imaging up to 3 mice, the φ-eye is a complete solution for all laboratories at any research stage. 

The system is just 60cm x 60cm x 60cm in size, weighing less than 60kg. This makes it truly a desktop solution which can turn any space into an imaging lab. Despite its small footprint, the φ-eye has a customizable and expandable set of filters allowing users to image most well-known probes, as well as newly developed probes. 

The animal handling system ensures the health and wellbeing of the imaging subject, while the Visual|eyes software suite provides a fully integrated, easy to use, platform for both image acquisition and data analysis. 

Features & Benefits

Enables Bioluminescence (BLI), Fluorescence (FLI), and Cherenkov Luminescence Imaging (CLI)

One system can be used to image a variety of fluorophores, enzyme-substrate reactions, and photons emitted by Cherenkov radiation.

Customizable, and expandable filter set

Users are able to image most of the commonly used fluorophores with the system, as well as newly developed probes.

Small footprint (60cm x 60cm x 60cm), and weight less than 60kg.

With its small size, and weight, the system is truly a benchtop system.

Easy to use acquisition software including pre-defined imaging protocols, and the ability to save user defined protocols as well.

New users can begin using the system quickly and easily; while advanced users can optimize their imaging protocols to suite their specific needs.

Anatomical mapping using artificially generated X-ray images.

The system does not product radiation, as it does not have an X-ray source; but anatomical information is still provided by the generated X-ray image. 

Active field of view of 17cm x 17cm

This field of view is suitable for whole body imaging of up to 3 mice, allowing for high throughput imaging.

White light excitation source

Provides excitation at all wavelengths, as well as superior photographic images.

Simple connections:
  • Electrical requirements – 100-240 VAC 
  • PC connectivity – USB 2.0 Type A and Gb Ethernet 

Allow the system to be positioned within any laboratory, animal facility, or clean room space – converting it into an imaging lab.

Easy to use analysis software and export capabilities including DICOM filed.

Users can quickly and easily generate quantitative data on user defined ROIs within the analysis software. Or users can export the data and perform analysis in third party software.

Animal handling system with integrated anesthesia delivery, and heated bed.

The user has the ability to maintain a stable physiological state of the imaging subject throughout the session. This allows consistency in data, as well as being important for the health and wellbeing of the animal.

Common imaging bed for all eye systems

Having a common imaging bed allows an animal to move from one eye system to another without moving. Images can be co-registered, allowing for multimodal imaging.

Models & Specifications

  • Detector – sCMOS
  • Resolution – 6.5 micron pixels, 2048×2048
  • Quantum Efficiency – 8.2% @ 560nm
  • Dark Current – 0.6 electrons/pixel/s
  • Dynamic Range – 33000:1
  • Lens – F/1.8; >93% transmittance (VIS-NIR)
  • Fluorescence bandpass filters – >93% transmission; >OD 6 blocking
  • Integrated anesthesia
  • Heated animal stage
  • Light tight chamber is 30cm x 30cm x 30cm
  • Capable of BLI, FLI, CLI
  • Visible range from 400-800nm
  • Customizable and expandable filter set – standard is 4 filters
  • Excitation with white light
  • Image up to 3 mice
  • Active field of view is 17cm x 17cm

 

Φ-eye Imaging Gallery

Applications

Cancer Research

BLI, FLI, and/or CLI can be used in cancer research applications in many ways:

  • Confirm the presence of tumors
  • Monitor the growth/size of tumors
  • Detect metastasis
  • Detect the expression of specific biomarkers

This image shows tumors on the flanks of each mouse expressing TdTomato, using fluorescence imaging.

Biomarker Detection

Targeted probes can be used to image specific biomarkers, for example:

  • Inflammation
  • Angiogenesis
  • Hypoxia
  • Etc.

Pharmacokinetics/Dynamics/Biodistribution

Labelled compounds can be used to detect the pharmacokinetics, pharmacodynamics, and biodistribution of the compound over time after administration to the imaging subject.

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