Cardiac function is the product of myocardial contractility, filling volume and vascular resistance. Abnormal cardiac function is characteristic of multiple disease states, and can be assessed using different in vivo and in vitro methods. We offer several solutions for the assessment of cardiac function, with options for acute or chronic measurements, invasive or non-invasive in vivo measures, or ex vivo preparations.
Pulsed Wave Doppler Assessment of Cardiac Function
The Doppler Flow Velocity System enables assessment of both systolic and diastolic function. Non-invasive Doppler probes can detect flow velocities in the heart and aorta as well as the peripheral vasculature. The small handheld probe produces highly accurate measurements compared to echocardiography, and the system can be used as a surrogate to highly invasive pressure catheter techniques.
Telemetry enables long-term data sampling in conscious, untethered animals. Indus Instruments telemetry systems enable ECG monitoring and recording in mice and rats, as well as temperature and activity. Kaha Sciences telemetry systems enable ECG monitoring and recording in mice and rats, as well as pressure and sympathetic nerve activity in rats. Both systems feature rechargeable batteries and the option to cohouse animals.
High frequency ultrasound may be used to assess cardiac function in small animals. B- and M-Mode images are used to assess ejection fraction, fractional shortening, volume changes, and LV mass. Color and PW Doppler are used to assess flow velocities within the heart and surrounding vasculature, while tissue Doppler is used to assess changes at the mitral annulus.
PET/CT Imaging combines the functional nuclear imaging of PET with the precision of an x-ray CT scanner to produce accurate anatomic renderings allowing for visualizing metabolic or biochemical activity within the body. From a cardiovascular standpoint, PET/CT is a vital tool used for quantification of cardiac perfusion and assessment of coronary anatomy which has previously been underutilized due to costs and lack of readily available equipment.
For non-invasive studies, we offer a tail cuff blood pressure system for rapid, repeatable blood pressure measurements over the course of a longitudinal study. Systems can be configured for multiple animals enabling high throughput screening. High-fidelity pressure-tipped catheters and implantable telemetry solutions enable intravascular and intraventricular blood pressure measurements.
ECG is a rapid, reliable way to detect electrical abnormalities in the heart. The Rodent Surgical Monitor+ (RSM+) enables non-invasive ECG acquisition in anesthetized animals and can be integrated with data acquisition systems. Telemetry options for mice and rats are also available for chronic ECG monitoring and recording, and the CardioFish system can be used to assess ECG in adult zebrafish.
Cardiac MR Imaging
MRI is the gold standard in soft tissue imaging. The M-seriesTM compact MRI system provides anatomical and morphological images to pre-clinical researcher focused on a variety of disease models including cancer, neurology, cardiovascular, inflammation, obesity & diabetes, as well as specific organ diseases and developmental biology.
Isolated heart perfusion systems are ideal for ex vivo assessment of cardiac function. We offer gravitational, pump-controlled and compact systems for mouse, rat, guinea pig and rabbit preparations. These systems can be configured as the Janicki-type Langendorff, where the heart is perfused through the aorta and the backward pressure forces flow into the coronary vessels, or the Neely-type working heart model.