What oxygen levels are you using in your cellular research? Are your cells under hyperoxia, normoxia, hypoxia, or are you unsure?
This free webinar hosted by Scintica Instrumentation provided our audience with an overview on the effects of oxygen during in vitro cell culturing. Further, the discussion focused on how to accurately determine and regulate the O2 levels during experiments.
Oxygen influences multiple physiological parameters within cells. As such, oxygen concentration should at least be considered when planning cell culture studies. In addition to monitoring and controlling for CO2, humidity and temperature, scientists should consider oxygen levels. Assessing oxygen concentration for all in vitro studies improves both scientific reproducibility and relevant outcomes by removing a potential confounding factor.
This webinar built on these concepts and discussed the importance of oxygen in cell culture. Dr. Pansters described a better method for defining O2 concentration, O2 monitoring and controlling options. He also provided a brief overview of relevant scientific publications. Participants gained a better understanding of how accurate oxygen regulation will contribute to improved reproducibility of their experimental work. Finally, participants learned what next steps exist to improve future research outputs.
Topics discussed in this webinar included:
- The meaning of hyperoxic, normoxic and hypoxic conditions at the cellular level
- How hyperoxic conditions affect the HIF1 transcription factor and its many downstream effects
- How oxygen is beginning to play a larger and larger role in cellular labs
- The future of hypoxic/normoxic cell culturing in research
If you want more information on our hypoxia options visit our Hypoxia Chambers page
Nicky Pansters, Ph.D.
Nicky holds a Ph.D. from the University of Maastricht, in the Netherlands. His expertise includes skeletal muscle remodeling, cell culture and molecular biology. He previously worked on cellular signaling pathways involved in muscle (cell) differentiation and (re)growth both in vitro and in vivo. Nicky is currently a product manager for Europe with Scintica Instrumentation.