The survival of tissues and organs relies on a sufficient supply of oxygen, among other things, which are distributed via microvascular blood perfusion. Tissue oxygen (pO2) is a readout of oxygen availability at the cellular level representing the balance between oxygen supply and metabolic oxygen consumption. In addition, the measurement of microvascular blood perfusion provides critical information for research applications where blood supply has been disrupted. Combining these measurements into one sensor gives researchers a very powerful and unique tool to answer questions in the areas of physiology, oncology, cerebral monitoring, ischemia/reperfusion, ophthalmology and many more. Methods used to measure these parameters are made possible using the latest in fiber optic sensing technology; the OxyLite™ and OxyFlo™ tissue vitality monitors.
The ability to assess microvascular blood flow and tissue oxygen concentrations is especially useful when investigating stroke and shock models, among a variety of other research applications that involve ischemia/reperfusion. Microvascular flow data can indicate occlusion, as observed in stroke/MI models, or microcirculatory health of individual organs, as demonstrated in some shock studies. Further, measuring dissolved oxygen can provide more meaningful data than blood oxygen saturation. Dissolved oxygen in the tissue, for example, indicates unbound oxygen available to cells. In contrast, there are a variety of factors that influence oxygen-hemoglobin dissociation and quantifying the percent of hemoglobin bound to oxygen (pulse oximetry, SpO2) may not be indicative of what is immediately available to the tissue. Using the OxyFlo and OxyLite probes, researchers can quantify tissue blood flow and dissolved oxygen with a wide range of probe sizes appropriate for a substantial list of applications.
During this webinar, Sarah McFarlane will discuss some of the applications for this state-of-the-art technology in pre-clinical research and explain the technology behind these sensors that allows researchers to measure both tissue oxygen and blood perfusion from specific microregions.