Intravital microscopy is a technique that enables you to directly observe the movement of live cells that make up living tissue in vivo. With this technique, it is possible to distinguish an individual cell among a large number of cells in various organs, which is not possible with conventional biomedical imaging technologies such as MRI or CT, and to track the movement of each cell in three dimensions in real-time. IVIM Technology’s All-in-One IntraVital Microscopy (IVM) system is extensively optimized and carefully engineered to provide superb performance in the intravital imaging of live animal models in vivo. It is the world’s first system to fully integrate body temperature regulation and anesthesia into the animal handling stage. The system also includes state-of-the-art live tissue motion compensation for improved image quality, along with ultrafast imaging speeds. The system may be configured for confocal or two/multi-photon microscopy, using either solid-state or femto-second pulsed lasers, respectively.

IVIM Technology was founded on the innovative technology of IntraVital Microscopy (IVM) developed by the Korea Advanced Institute of Technology (KAIST). First founded in 2017, IVIM Technology released the IVM-C and IVM-CM models in 2018. The IVM-M and IVM-MS models were released later in 2019. In mid-2020, a US-based reference site was established at Harvard Medical School in Boston, MA.

Intravital microscopy enables dynamic, 3D, cellular-level imaging of various biological processes in vivo. It enables scientists to directly verify hypothesis derived from ex vivo or in vitro observations in natural physiological in vivo microenvironments.

Using intravital microscopy, in vivo visualizations of gene expression, protein activity, cell trafficking, cell-cell / cell-microenvironment interactions, and various physiological responses to stimuli have been accomplished, providing novel insights, which have been impossible to obtain with conventional static 2D observation of ex vivo or in vitro samples.

However, up until now, individual users have had to improvise the required functions for each of the intravital imaging applications for the various organs with conventional standalone microscopes, which resulted in non-optimal performance for limited applicability.

The IntraVital Microscopy (IVM) systems from IVIM Technology, is a fully integrated system with optimized optical, mechanical, and electrical components to ensure best-in-class imaging performance for a wide range of in vivo intravital imaging applications.

The IVM systems (IVM-C/M/CM/MS) provide an all-in-one in vivo imaging solution providing integrated physiological monitoring and support, anesthesia, along with state-of-the-art living tissue motion compensation for improved image quality. The software has been fully optimized to allow for rapid image acquisition, while image processing is accelerated by GPU-assisted parallel computing.

FeatureBenefit
Integrated temperature support, and physiological monitoringEnsures animal well-being throughout the imaging session, and consistency between animals within a study

Integrated inhaled anesthesiaEnsures ease of animal handling and consistent plane of anesthesia
Animal motion compensationProvides enhanced image quality on organs which may be affected by respiratory motion

User friendly design – both software and hardwareAllows for ease of use, and reproducible results
4-color simultaneous imagingConfocal / Two-Photon Modes – Allows users to track the movement of several cells in vivo to better understand the biological processes being examined
Ultrafast rotating polygonal mirror scanner
  • Allows for ultrafast uniform laser-beam scanning with uniform excitation illumination over the entire field of view (FOV)
  • Enables ultra-high speed in vivo imaging (max. 100fps @ 512×512 pixels FOV)

IVM-C (Confocal)
  • IVM-C is optimized for in vivo imaging experiments. It is specially equipped with four (4) different wavelength lasers and four (4) high-sensitivity confocal detectors. IVM-C is the optimal system to observe highly diverse, dynamic multi-cellular behaviors in living animals simultaneously with four (4) different fluorescence colors.
    • Easy and highly efficient multi-color simultaneous imaging
    • High flexibility in the selection of laser/detector wavelength
    • Customizable design for modifications according to the customer’s requests and accommodations for future updates
    • Applicability for conventional ex vivo, in vitro, and in vivo imaging
IVM-M (Two-Photon)
  • IVM-M is optimized for in vivo imaging experiments. Especially because it is equipped with a fully-automated tunable femtosecond (fs) pulse NIR laser system. IVM-M is the optimal system for users who need to conduct deeper-tissue imaging using less-scattering NIR wavelengths. The fs-laser system’s full control functionality is integrated with the two-photon imaging software for user convenience with various automated algorithms.
    • Laser wavelength tuning for optimal two-photon excitation of a wide-range of fluorescence agents
    • Deeper tissue imaging with a longer-wavelength NIR fs-laser system
    • Capable of label-free, non-linear multi-harmonic generation imaging
    • Motion compensation and ROI tracking for long-term in vivo imaging
IVM-CM (Confocal & Two-Photon)
  • IVM-CM is optimized for in vivo imaging experiments. Especially because both the confocal and two-photon microscopes are integrated into a single-box packaged system. IVM-CM provides the ultimate versatile functionality of the IVM-C and IVM-M in one system
    • Dual-mode IVM-C (confocal) / IVM-M (two-photon) imaging capability
    • One-click automated transition between confocal and two-photon imaging mode
IVM-MS (Two-Photon Smart Version)
  • IVM-MS is optimized for in vivo imaging experiments and is equipped with a new compact, high-efficiency femtosecond (fs) pulse laser module. Especially because it integrates a compact high-stability maintenance-free fs-pulse laser into a single box. The IVM-MS is the ideal solution for customers in need of a two-photon microscope with limited resources of space and budget.
    • Less expensive, fully-automated fs-laser system
    • Space-saving with no additional fs-laser box configuration
    • Simple hand-free turn-key operation of the fs-laser for two-photon excitation
    • Easy maintenance and management without liquid cooling system requirements

Confocal Microscopy

  • High-resolution optical sectioning imaging of in vivo tissue
    • Rejection of fluorescence signal from out-of-focus volume by confocal pinhole
    • High contrast, low background, high quality in vivo imaging
  • Ultrafast precise Raster scanning of multi-color excitation laser-beam focus
    • Video-rate imaging of fast cellular-level dynamics in live tissue in situ
  • Ideal for high-sensitivity multi-color intravital imaging of in vivo tissue


Two-Photon Microscopy

  • High-resolution optical sectioning imaging of in vivo tissue
    • Fluorescence signal is intrinsically generated only at the focus
  • Deeper tissue imaging with longer-wavelength near-infrared (NIR) fs-pulse laser for two-photon / multi-photon excitation
  • Capable of label-free, non-linear multi-harmonic generation imaging (SHG, THG)
  • No photo-bleaching at non-focal plane; reduced phototoxicity
  • Ideal for long-term 3D intravital imaging of in vivo tissue


Confocal MicroscopyTwo-Photon Microscopy
  • Single-photon excitation
  • Two-photon excitation
  • Point scanning + pinhole
  • Point scanning + no pinhole
  • Optical sectioning:
    • Fluorescence signal from out-of-focus is blocked
  • Optical sectioning:
    • Fluorescence signal is intrinsically generated only at the focus
  • Imaging depth – 100-200µm
  • Imaging depth – 250-1000µm
  • Continuous-wave solid-state laser with flexible choice of wavelengths at the range from ultraviolet (UV) and visible (VIS) to near-infrared (NIR)
  • Femto-second pulsed laser tunable at near-infrared (NIR) wavelength range
  • Descanned confocal detector
  • Non-descanned detector (NDD)
  • Easy and efficient, multi-color 3D intravital imaging
  • Deeper-tissue 3D intravital imaging

Applications

The IVM series of systems have been designed to provide expandability and flexibility for highly diverse intravital imaging applications of various tissues and organs, including the brain, liver, spleen, kidney, pancreas, lung, heart, gastrointestinal tract, retina, skeletal muscle, bone marrow, peripheral lymph node, prostate, thymus, thyroid gland, adipose tissue, blood, and lymphatic vessels, etc.

In addition, to handle various imaging needs raised by researchers for a wide range of biomedical studies, detailed experimental protocols have been established for high-quality intravital image acquisition.

Subsequent quantitative analysis of various cellular-level dynamics and physiological alterations have been established.

  • In Vivo Live Cell Imaging
    • IVM systems allow for dynamic 3D imaging of various cellular-level dynamics such as cell trafficking, cell-cell interaction, cell-microenvironment interactions in vivo; providing insights into the processes of human disease progression and response to therapeutic interventions
  • Drug Discovery
    • The IVM series of systems serve as a highly valuable and versatile tool allowing:
      • Target identification and validation in natural in vivo microenvironments
      • Confirmation of mode of action (MoA) and proof of concept (PoC) of new therapeutics by direct in vivo cellular observation
      • Monitoring of in vivo drug delivery, distribution, retention and in situ efficacy at target tissues and organs
    • Select Imaging Examples
      • Inguinal Adipose Tissue

      • Neuromuscular Junction

      • Immune Cell Imaging

      • Nanoparticle Imaging in the Liver

      • Retinal Imaging


      • Xenograft Imaging

Accessories and Add-ons

The various IVM models have the following optional add-on items:

  • Integrated inhaled anesthesia system
  • Optional high (60fps) or ultra-high (100fps) speed scanning module
  • Optional objective lens set for two-photon or confocal imaging
  • In vivo imaging chamber
  • Lung imaging chamber
  • Cranial window
  • Abdominal window
  • Pancreas imaging window
  • Mammary imaging window