IntraVital Microscopy is an all-in-one confocal/two-photon microscopy system designed and optimized for longitudinal imaging of live animal models in vivo. This is the first all-in-one intravital microscopy platform that can explore complex dynamic behaviors of numerous cells in a living body and serve as a next-generation core technology to elucidate unknown pathophysiology of various human diseases. Confocal capabilities of the IVM system enables optical sectioning of in vivo tissue via rejection of out-of-focus fluorescence light coming from the background tissue which will result in images with high contrast and quality. In addition to confocal, IVM systems are equipped with two-photon lasers which use longer-wavelength near-infrared (NIR) fs-pulse excitation capable of deep tissue imaging as well as label-free, non-linear multi-harmonic generation imaging (SHG, THG).
With this technique, it is possible to distinguish an individual cell among a large number of cells in various organs, which is not possible with conventional biomedical imaging technologies such as MRI or CT, and to track the movement of each cell in three dimensions in real-time. It is the world’s first system to fully integrate body temperature regulation and anesthesia into the animal handling stage. The system also includes state-of-the-art live tissue motion compensation for improved image quality, along with ultrafast imaging speeds. The system may be configured for confocal or two/multi-photon microscopy, using either solid-state or femto-second pulsed lasers, respectively.
IVIM Technology was founded on the innovative technology of IntraVital Microscopy (IVM) developed by the Korea Advanced Institute of Technology (KAIST). First founded in 2017, IVIM Technology released the IVM-C and IVM-CM models in 2018. The IVM-M and IVM-MS models were released later in 2019. In mid-2020, a US-based reference site was established at Harvard Medical School in Boston, MA.
Intravital microscopy enables dynamic, 3D, cellular-level imaging of various biological processes in vivo. It enables scientists to directly verify hypothesis derived from ex vivo or in vitro observations in natural physiological in vivo microenvironments.
Using intravital microscopy, in vivo visualizations of gene expression, protein activity, cell trafficking, cell-cell / cell-microenvironment interactions, and various physiological responses to stimuli have been accomplished, providing novel insights, which have been impossible to obtain with conventional static 2D observation of ex vivo or in vitro samples.
However, up until now, individual users have had to improvise the required functions for each of the intravital imaging applications for the various organs with conventional standalone microscopes, which resulted in non-optimal performance for limited applicability.
The IntraVital Microscopy (IVM) systems from IVIM Technology, is a fully integrated system with optimized optical, mechanical, and electrical components to ensure best-in-class imaging performance for a wide range of in vivo intravital imaging applications.
The IVM systems (IVM-C/M/CM/MS) provide an all-in-one in vivo imaging solution providing integrated physiological monitoring and support, anesthesia, along with state-of-the-art living tissue motion compensation for improved image quality. The software has been fully optimized to allow for rapid image acquisition, while image processing is accelerated by GPU-assisted parallel computing.
IVM-C is optimized for in vivo imaging experiments. It is specially equipped with four (4) different wavelength lasers and four (4) high-sensitivity confocal detectors. IVM-C is the optimal system to observe highly diverse, dynamic multi-cellular behaviors in living animals simultaneously with four (4) different fluorescence colors.
Easy and highly efficient multi-color simultaneous imaging
High flexibility in the selection of laser/detector wavelength
Customizable design for modifications according to the customer’s requests and accommodations for future updates
Applicability for conventional ex vivo, in vitro, and in vivo imaging
IVM-M is optimized for in vivo imaging experiments. Especially because it is equipped with a fully-automated tunable femtosecond (fs) pulse NIR laser system. IVM-M is the optimal system for users who need to conduct deeper-tissue imaging using less-scattering NIR wavelengths. The fs-laser system’s full control functionality is integrated with the two-photon imaging software for user convenience with various automated algorithms.
Laser wavelength tuning for optimal two-photon excitation of a wide-range of fluorescence agents
Deeper tissue imaging with a longer-wavelength NIR fs-laser system
Capable of label-free, non-linear multi-harmonic generation imaging
Motion compensation and ROI tracking for long-term in vivo imaging
IVM-CM (Confocal & Two-Photon)
IVM-CM is optimized for in vivo imaging experiments. Especially because both the confocal and two-photon microscopes are integrated into a single-box packaged system. IVM-CM provides the ultimate versatile functionality of the IVM-C and IVM-M in one system
One-click automated transition between confocal and two-photon imaging mode
IVM-MS (Two-Photon Smart Version)
IVM-MS is optimized for in vivo imaging experiments and is equipped with a new compact, high-efficiency femtosecond (fs) pulse laser module. Especially because it integrates a compact high-stability maintenance-free fs-pulse laser into a single box. The IVM-MS is the ideal solution for customers in need of a two-photon microscope with limited resources of space and budget.
Less expensive, fully-automated fs-laser system
Space-saving with no additional fs-laser box configuration
Simple hand-free turn-key operation of the fs-laser for two-photon excitation
Easy maintenance and management without liquid cooling system requirements
The IVM series of systems have been designed to provide expandability and flexibility for highly diverse intravital imaging applications of various tissues and organs, including the brain, liver, spleen, kidney, pancreas, lung, heart, gastrointestinal tract, retina, skeletal muscle, bone marrow, peripheral lymph node, prostate, thymus, thyroid gland, adipose tissue, blood, and lymphatic vessels, etc.
In addition, to handle various imaging needs raised by researchers for a wide range of biomedical studies, detailed experimental protocols have been established for high-quality intravital image acquisition.
Subsequent quantitative analysis of various cellular-level dynamics and physiological alterations have been established.
In Vivo Live Cell Imaging
IVM systems allow for dynamic 3D imaging of various cellular-level dynamics such as cell trafficking, cell-cell interaction, cell-microenvironment interactions in vivo; providing insights into the processes of human disease progression and response to therapeutic interventions
The IVM series of systems serve as a highly valuable and versatile tool allowing:
Target identification and validation in natural in vivo microenvironments
Confirmation of mode of action (MoA) and proof of concept (PoC) of new therapeutics by direct in vivo cellular observation
Monitoring of in vivo drug delivery, distribution, retention and in situ efficacy at target tissues and organs