Oxford Optronix - HypoxyLab - Internal connector for an OxyLite sensor

The survival of tissues and organs relies on an adequate supply of oxygen. Tissue oxygen tension (ptiO2) provides a direct measurement of the balance between oxygen supply (by the blood) and metabolic oxygen consumption (by the tissue), i.e. a readout of oxygen availability at the cellular level.

There are several ways researchers can measure in vivo tissue oxygen: electrode sensors, injectable/soluble phosphorescent probes, and fibre-optic phosphorescent probes. The advantage of phosphorescence quenching technology is it does not consume oxygen providing accurate measurements with minimal error while preserving spatial precision and accuracy. Within this this webinar is an introduction to the OxyLite by Oxford Optronix, a state-of-the-art tissue oxygen measurement system, providing accurate, real-time, dynamic recordings of pO2 in a variety of animal and organ/tissue models.

This webinar provided an introduction of:

– Tissue oxygenation and its importance
– Sensor technologies available for measuring tissue oxygen availability
– Fibre-optic tissue oxygen sensors
– Tissue Vitality Monitoring for in vivo and in vitro applications
– Highlight of interesting pO2 applications

Sydney Mensen, MSc

Sydney holds a Master of Management of Applied Sciences, specializing in Biological Sciences, and a double major in Biology and Medical Sciences—both from the University of Western Ontario. She has completed several courses and laboratory work involving anatomy and physiology of mammals. Her professional career has involved the application of her detailed understanding of mammalian physiology to obtain and analyze scientific data using a variety of technologies, including ultrasound, MRI, and surgical equipment. Her current role at Scintica is to work with scientists to understand their needs and provide technologies that will help to achieve their research goals.

Gus Kalogeros Ph.D.

Gus Kalogeros holds H.BSc. (1991) and a Ph.D. (1998) in Pharmacology and Toxicology, both from University of Western Ontario.  His Ph.D. studied the effects of anesthetics and hyperbaric environments on cytosolic calcium in neurons utilizing cellular fluorescence and infrared spectroscopy techniques.

In his previous role as Accounts Manager and Application Specialist with Sonometrics (1999-2020), he acquired extensive knowledge and experience in cardiovascular physiology and invasive experimental instrumentation.  In addition to becoming an expert in digital sonomicrometry, he also became proficient with intracardiac/intravascular pressuremetry, ultrasound flowmetry and cardiac volumetry.  He worked closely engineers and investigative researchers at medical device companies involved in the design of LVADs, stents and artificial cardiac valves, as well as pharmaceutical companies and institutional research facilities, to provide customized research solutions.

Presently, Gus is at Scintica Instrumention where he serves as Preclinical Product Manager with a focus on products for the cardiovascular physiology laboratory.  These products include Doppler flow velocity systems, high-frequency ultrasound, tissue blood/oxygen perfusion monitors, arteriograph/myograph systems and telemetry.  His role is to present these technologies to investigators and work with them to meet their research objectives.