Befitting this, laser confocal endomicroscopy (LCE), with its probe-based imaging approach, delivers cellular resolution, while being minimally invasive. Many scientists and imaging core facility managers refer to it as “virtual histology”; it captures real time microscale images like classical histology, in a non-destructive manner. It is a cutting-edge imaging modality with endless possibilities in preclinical imaging.
The FIVE2 (ViewnVivo) from Optiscan Imaging is the latest and most competent model of LCE designed for effortless, real-time preclinical imaging in numerous animal models. This live webinar from two different imaging experts provided scientists and postdocs with the theoretical and practical knowledge about the FIVE2 (ViewnVivo) and its preclinical research applications. Former product manager of FIVE2 technology, Dr. Mohammedayaz Rangrez, gave a hands-on demonstration of the technology, imaging in different tissue types, Z sectioning and other key capabilities of the system. Dr. Howard Dobson from Invicro discussed some of its applications and image analysis illustrated with examples from normal and diseased rodents. This included images of the liver, kidneys and colon. Imaging of the colon was demonstrated using an intraabdominal approach in both rats and mice, as well as through a rectal approach in rats, making it ideally suited for longitudinal studies. In addition, the use of the FIVE2 (ViewnVivo) to image and quantify blood flow in small vessels was illustrated. Examples of quantitative image analysis approaches were included for each organ.
Topics to be discussed in this webinar included:
- Hardware functionality and software operation of the FIVE2 (ViewnVivo) – fluorescence in vivo endomicroscope
- How to use the FIVE2 (ViewnVivo) for imaging tissue architecture with cellular resolution and for optical sectioning
- Examples of normal and abnormal tissues in the abdominal cavity using a variety of fluorophores
- Overview of the approach to image analysis
- An understanding of applications of the FIVE2 (ViewnVivo) in preclinical research