The mechanisms involved in the progression of heart failure includes cardiomyocyte death, initiation of the inflammatory response, and subsequent formation of a fibrotic scar. For this reason, diverse experimental models, such as cultured cardiac cells, isolated hearts, and animal models, have been created to mimic human myocardial infarction and heart failure. In a 2012 Scientific Statement from the American Heart Association, permanent coronary artery ligation was deemed clinically relevant and a significant model for ischemic heart failure.
The variability in coronary artery anatomy and branching of the left coronary artery can affect reproducibility of MI surgery, even with occlusion at a constant anatomic site. Blanching and LV discoloration are common variables used to confirm successful MI surgery. Determining a way to improve precision will improve the standard surgical approaches to simulate MI in mice, thus reducing the number of experimental animals necessary for statistically relevant studies. In this webinar, we discussed the advantages of using electrocardiography to verify correct ligation.
- Summary of my lab goals
- Timeline of inflammation after permanent occlusion
- Intro to MI surgical model
- EKG measurement (changes before and after MI)