The Newton 7.0 is an innovative optical bioluminescence and fluorescence imaging system designed with the user in mind. It is ideal for in vivo, ex vivo and in vitro imaging applications, allowing for simultaneous imaging of multiple animals or samples at a time. It’s advanced features and software are easy to navigate and optimized for a multi-imager interface. Furthermore, the user-friendly workflow and advanced system sensitivity facilitates time-saving signal acquisition for longitudinal studies.

VILBER is a leading life science company developing and manufacturing fluorescence, chemiluminescence and bioluminescence imaging systems for applications ranging from small animal to cell biology research. Founded in 1954, Vilber is a leader in the molecular imaging sector, equipping more than 20,000 laboratories worldwide. An estimated 60,000 people use their products every day in over 100 countries.

The NEWTON 7.0 is a highly sensitive optical imaging system dedicated to pre-clinical imaging of small animals in vivo. It combines the best optics and animal
handling features for optimum scientific images and results.

  • User-friendly instrument
  • No radiation
  • Longitudinal studies / Non-invasive
  • Large throughput studies / 5 Mice capacity
FeatureBenefit
Powerful Fluorescence ExcitationThe Spectra Light Capsules are powerful excitation sources that effectively focus light for uniform illumination and enhanced power. Equipped with primary and secondary optics, the Light Capsules are categorized as Laser Class II due to their intense power.

Benefit from an excellent signal to background ratio for imaging and sensitivity to detect the lowest signal concentration possible.

Full Spectrum Tunability8 excitation sources and 10 emission filters are available to cover the complete spectrum from Blue to infra-red.

Only 40nm separates each wavelength, where each Spectra Light Capsule produces a monochromatic light of a specific wavelength with a very narrow-band illumination.

This reduces the cross stimulation and increases the sensitivity of your images.

Macro-imaging to large throughput studiesVilber’s innovative darkroom architecture allows you to position your samples or animals at various levels in the Z-axis depending on the sample size.
10 Megapixels image resolutionHigh Resolution CCD camera
Peltier Thermoelectric coolerDeep CCD Cooling (-90˚C) for optimal signal to noise ratio
Motorized V.070 lens: f:0.70Widest lens aperture Highly sensitive
4.8 Optical DensityExtremely linear over dynamic range of signal detection

Easily detect fluorescent molecules and reporters at the picogram level in your tissue of interest using Vilber’s dynamic range of excitation light capsules and emission spectra.

For deeper tissue penetration and reduction of autofluorescence background, infrared (IF) and near-infrared (NIR) molecules can also be imaged with Vilber.

Bioluminescence imaging can be used to detect luciferase-tagged molecules at the femtogram level.

                                                                         

Perform monitoring of various fluorescent signals by multi-color in vivo imaging.

Signals can be overlaid so that several reporters can be visualized simultaneously.

Images acquired at different time points can be arranged to form a longitudinal image sequence. For example, a time series could be constructed from images acquired on different days following an experimental treatment.

The software then compares the image data throughout the experimental treatment.

 
           Day 1                               Day 5                             Day 10                              Day 15                           Day 20                          Day 25                             Day 30                            Day 35

NiraWave M is a micellar formulation of the clinically-tested NIR fluorescent dye Indocyanine Green. Due to the unique formulation, NiraWave M exhibits intense fluorescence, higher aqueous stability and prolonged blood circulation time. It is optimized for fluorescence angiography, particularly for the visualization of the microcirculation.

Highlight vascular structures and benefit from this bright and long-lasting vascular contrast agent. While in the circulation, the high aqueous stability prevents rapid fluorescence decay of the ICG molecule.

                                     

NiraWave Rocker is a nanoparticle formulation of a NIR fluorescent imaging agent optimized for investigating vascular leakage within inflamed tissues and tumors. Assess tissue permeability and retention (EPR) effect using NiraWave Rocker, and also track intracellular accumulation of the agent in certain cell types.

Highlight inflamed tissues and tumors and benefit from NIR deep tissue penetration.

Apply NiraWave Rocker to:

  • Detect areas of increased vascular permeability
  • Study inflammatory processes such as in rheumatic diseases
  • Monitor therapeutic responses of affected tissues

3D Dynamic Scanning Mode provides a live 3D representation of the optical signal to localize labeled tissue and tissue distribution of signal concentration.

Inflammatory responses can be assessed using non-invasive fluorescence biomarker imaging in a preclinical model of rheumatoid arthritis.

Using the Vilber imaging system, researchers investigated the biodistribution of doxorubicin hydrochloride-loaded nanogels in rats (Sprague–Dawley, 220–250 g). The fluorescent signal of DOX was detected and monitored over eight hours, seen below.


The major organs were dissected 10 h after oral administration and observed ex vivo.

Signal quantification demonstrated that organs harvested from rats treated with doxorubicin hydrochloride-loaded nanogels group exhibited significantly higher retention of doxorubicin hydrochloride compared to rats treated with doxorubicin hydrochloride alone.


In vivo fluorescence imaging of FITC-BSA nanoparticle in the Turbot fish.

After 36 h, the heart and liver were dissected and visualized nanoparticle retention was identified.

                           
Distribution of FITC-BSA in turbot fish at different time points                                                                          In vivo fluorescence imaging of FITC-BSA distribution in organs at 36 h

Bioluminescent enterotoxic E. coli (ETEC) is tracked through the mouse intestine, demonstrating the colonization dynamics across the GI tract.

Streptomycin-treated BALB/c mice were inoculated with E. coli bacteria via gavage with pRMkluc-tagged ETEC and pRMkluc-tagged E. coli K-12.  Luciferin was administered intraperitoneally prior to imaging.

After inoculation, bioluminescence was localized to the small intestine. 48 hours post-inoculation, the bioluminescent signals indicated bacterial passage through the mouse intestine. Bioluminescent signals were detected in the mouse intestine up to 120 h post-inoculation.

After 120 h of E. coli infection, mouse gastrointestinal tracts were extracted to perform ex vivo imaging. Intestinal tract dissection included the esophagus to rectum.

ETEC were localized in the proximal mouse ileum approximately 6 cm from the cecum, whereas the E. coli K-12  ol signals were identified in the cecum and in the proximal colon.

BALB/c mice were intraperitoneally inoculated with E. coli K-12 tagged with pRMkluc or E. coli K-12 tagged with pBR322 (incubated with luciferin).

After 1 h of infection, bioluminescent signal emission from the animals was captured.

Mice infected with E. coli K-12 harboring pBR322 did not exhibit bioluminescent emission (Fig.B). However, mice infected with E. coli harboring pRMkluc emitted bioluminescent signals detected in the mouse inoculation zone (Fig.A).


in vivo bioluminescence emission of E. coli K-12

Screen localization dynamics of fluorescently-tagged drugs. Investigators injected a Cy5.5-tagged drug and tracked it’s spread systemically in the mouse.

Tumor progression can be monitored after establishment of orthotopic tumors in mice. The mouse brain was injected with 10,000 (fig.a ) and 50,000 (fig.b ) cancer cells expressing luciferase.

After a 6 weeks, tumors were formed, where signal was dependent on tumor cell injection quantity.

                                                                                   

Fig a: 10 000 tumoral cells injected in the brain                                                                                                      Fig a: 50 000 tumoral cells injected in the brain

SystemNewton Mini 7.0Newton 7.0 FT400
Optics
Camera16-bit Scientific Grade CCD
CCD Cooling-90 C
Image Resolution10 Megapixels
LensFixed Focal Length f/0.7 Motorized
Excitation and Emission
Epi-FluorescenceOptional individual channels8 Channels included
Emission filtersOptional narrow bandpass filters9 Filters included
Filter Wheel7-position Motorized10-position Motorized
Darkroom Architecture
CameraFixedZ-Axis Motorized
StageHeight Adjustable (manually)X/Y-Axis Motorized
Animal Capacity3 mice / 1 rat5 mice / 3 rats
Animal Accessories
Heated bed+37°C thermoregulated bed included
Animal breathers3 or 5 animal breathers with nose cones included
WAGWaste Gas Scavenger included
AnesthesiaBiosthesia compatible (optional)

Supported by prestigious publications

Peng Xue , Mengmeng Hou, Lihong Sun et al.
Calcium carbonate packaging magnetic polydopamine nanoparticles loaded with indocyanine green for near infrared […] therapy
Acta Biomaterialia 10.1016/j.actbio.2018.09.045

Xiu Jun Fu, Yun Qing Zhu, Yin Bo Peng et al.
Enzyme activated photodynamic therapy for methicillin resistant Staphylococcus aureus infection both in vitro and in vivo
Journal of Photochemistry and Photobiology B: Biology 10.1016/j.jphotobiol.2014.04.016

Peng, Yinbo , Chenlu Song, Chuanfeng Yang et al.
Low molecular weight chitosan coated silver nanoparticles are effective for the treatment of MRSA infected wounds
International Journal of Nanomedicine 10.2147/IJN.S122357

Gerardo E. Rodea , Francisco X. Montiel Infante , Ariadnna Cruz Córdova et al.
Tracking Bioluminescent ETEC during in vivo BALB/c Mouse Colonization
Front Cell Infect Microbiol. 10.3389/fcimb.2017.00187

Huizi Keiko Li, Yukie Morokoshi , Kotaro Nagatsu et al.
Locoregional therapy with α‐emitting trastuzumab against peritoneal metastasis of human epidermal growth factor receptor 2‐positive gastric cancer in mice
Cancer Science 10.1111/cas.13282

Ming Kong, Lin Hou, Juan Wang et al.
Enhanced transdermal lymphatic drug delivery of hyaluronic acid modified transfersomes for tumor metastasis therapy
Chemical Communications 10.1039/c4cc08746a

Chao Feng, Guohui Sun, Zhiguo Wang et al.
Transport mechanism of doxorubicin loaded chitosan based nanogels across intestinal epithelium
Europ . J. of Pharmaceutics and Biopharmaceutics 10.1016/j.ejpb.2013.11.007

Qian Li, Lihong Sun, Mengmeng Hou et al.
A Phase change Material Packaged within Hollow Copper Sulfide Nanoparticles Carrying Doxorubicin and Chlorin e6 for Fluorescence guided Trimodal Therapy
of Cancer
ACS Appl. Mater. Interfaces, 10.1021/acsami.8b19667

Peng Xue , Mengmeng Hou, Lihong Sun et al.
Calcium carbonate packaging magnetic polydopamine nanoparticles loaded with indocyanine green for near infrared induced photothermal/photodynamic
therapy
Acta Biomaterialia 10.1016/j.actbio.2018.09.045