The Newton 7.0 is an innovative optical bioluminescence, fluorescence, and 3D tomographic imaging system designed with the user in mind. It is ideal for in vivo, ex vivo and in vitro imaging applications, allowing for simultaneous imaging of multiple animals or samples at a time. It’s advanced features and software are easy to navigate and optimized for a multi-user interface. Furthermore, the intuitive workflow and advanced system sensitivity facilitates time-saving signal acquisition for longitudinal studies.

VILBER is a leading life science company developing and manufacturing fluorescence, chemiluminescence and bioluminescence imaging systems for applications ranging from small animal to cell biology research. Founded in 1954, Vilber is a leader in the molecular imaging sector, equipping more than 20,000 laboratories worldwide. An estimated 60,000 people use their products every day in over 100 countries.

The Newton 7.0 is a highly sensitive optical imaging system dedicated to pre-clinical imaging of small animals in vivo, and may also be used on a variety of in vitro and ex vivo samples. It combines the best optics and animal handling features for optimum scientific images and results. The Newton 7.0 systems are capable of bioluminescence, fluorescence as well as 3D tomographic imaging. The system is:

  • User-friendly
  • Does not require any radiation to acquire images
  • Is non-invasive, allowing for longitudinal studies
  • Allows for up to 5 mice or 3 rats to be imaged simultaneously
FeatureBenefit
Powerful Fluorescence ExcitationThe Newton 7.0 offers 8 excitation channels in the visible RGB and near infrared spectrums. The very tight LED spectrum is additionally constrained with a very narrow excitation filter; these excitation sources are categorized as a Laser Class II due to their intense power. Movement of the excitation source over the entire FOV ensures consistent and reproducible results over the course of a longitudinal study
Full Spectrum Tunability8 excitation channels and 8 emission filters are available to cover the complete spectrum from Blue to infra-red.

Narrow bandpass filters are used for both excitation and emission to reduce cross talk between dies, allowing for up to 3 dyes to be imaged simultaneously

Macro-imaging to large throughput studiesVilber’s intelligent darkroom architecture allows for fully automated movement of the camera (Z-axis) and animal pad (X/Y axis) to move through both the macro imaging FOV (6x6cm) to the full FOV (20x20cm) for imaging up to 5 mice
Spectral UnmixingSpectral unmixing is possible for both bioluminescence and fluorescence imaging when different luciferase enzymes or fluorescent dies are used.

The includes algorithms to remove crosstalk between the different signals, allowing for each channel to contain signal from only one reporter

3D Optical TomographyAn integrated 3D tomography module allows both bioluminescence and fluorescence signals to be reconstructed in 3D and overlaid within a topographical model of the imaging subject.

For better understanding of anatomical and deeper tissue structures, the digital organ library allows for superimposition of the mouse organs and bones onto the topographical model

State-or-the-art camera technology:

  • Scientific grade 16-bit CCD
  • -90oC delta cooling
  • f/0.7 aperture
  • 10 megapixel image resolution
  • 4.8 Optical Density
The advanced camera and optics provide increased sensitivity to either bioluminescence or fluorescence signals, with a very low signal to noise ratio. The high optical density allows for samples with both very low and high signals to be imaged without saturation, allowing for quantifiable results

Easily detect fluorescent molecules and reporters at the picogram level in the tissue of interest using Vilber’s dynamic range of excitation emission wavelengths.

For deeper tissue penetration and reduction of autofluorescence background, infrared (IF) and near-infrared (NIR) molecules can also be imaged with Vilber.

The below dyes can be used with the Newton 7.0 systems:

Bioluminescence imaging can be used to detect luciferase-tagged molecules at the femtogram level.

                                                                         

Multispectral in vivo imaging is possible by using different luciferase enzyme/substrate pairs or by using different fluorescent dyes.

Signals can be overlaid within the same image, up to 3 reporters can be imaged simultaneously.

Images acquired at different time points can be arranged to form a longitudinal image sequence. For example, a time series could be constructed from images acquired on different days following an experimental treatment.

The software then compares the image data throughout the experimental treatment.

 
           Day 1                               Day 5                             Day 10                              Day 15                           Day 20                          Day 25                             Day 30                            Day 35

Inflammatory responses can be assessed using non-invasive fluorescence biomarker imaging in a preclinical model of rheumatoid arthritis.

Using the Vilber imaging system, researchers investigated the biodistribution of doxorubicin hydrochloride-loaded nanogels in rats (Sprague–Dawley, 220–250 g), click here for the link to the article abstract. The fluorescent signal of DOX was detected and monitored over eight hours, seen below.


The major organs were dissected 10 h after oral administration and observed ex vivo.

Signal quantification demonstrated that organs harvested from rats treated with doxorubicin hydrochloride-loaded nanogels group exhibited significantly higher retention of doxorubicin hydrochloride compared to rats treated with doxorubicin hydrochloride alone.


In vivo fluorescence imaging of FITC-BSA nanoparticle in the Turbot fish.

After 36 h, the heart and liver were dissected and visualized nanoparticle retention was identified.

                           
Distribution of FITC-BSA in turbot fish at different time points                                                                          In vivo fluorescence imaging of FITC-BSA distribution in organs at 36 h

Bioluminescent enterotoxic E. coli (ETEC) is tracked through the mouse intestine, demonstrating the colonization dynamics across the GI tract. Click here for the link to the article abstract

Streptomycin-treated BALB/c mice were inoculated with E. coli bacteria via gavage with pRMkluc-tagged ETEC and pRMkluc-tagged E. coli K-12.  Luciferin was administered intraperitoneally prior to imaging.

After inoculation, bioluminescence was localized to the small intestine. 48 hours post-inoculation, the bioluminescent signals indicated bacterial passage through the mouse intestine. Bioluminescent signals were detected in the mouse intestine up to 120 h post-inoculation.

After 120 h of E. coli infection, mouse gastrointestinal tracts were extracted to perform ex vivo imaging. Intestinal tract dissection included the esophagus to rectum.

ETEC were localized in the proximal mouse ileum approximately 6 cm from the cecum, whereas the E. coli K-12  ol signals were identified in the cecum and in the proximal colon.

BALB/c mice were intraperitoneally inoculated with E. coli K-12 tagged with pRMkluc or E. coli K-12 tagged with pBR322 (incubated with luciferin). Click here for a link to the article abstract.

After 1 h of infection, bioluminescent signal emission from the animals was captured.

Mice infected with E. coli K-12 harboring pBR322 did not exhibit bioluminescent emission (Fig.B). However, mice infected with E. coli harboring pRMkluc emitted bioluminescent signals detected in the mouse inoculation zone (Fig.A).


in vivo bioluminescence emission of E. coli K-12

Screen localization dynamics of fluorescently-tagged drugs. Investigators injected a Cy5.5-tagged drug and tracked it’s spread systemically in the mouse.

Tumor progression can be monitored after establishment of orthotopic tumors in mice. The mouse brain was injected with 10,000 (fig.a ) and 50,000 (fig.b ) cancer cells expressing luciferase.

After a 6 weeks, tumors were formed, where signal was dependent on tumor cell injection quantity.

                                                                                   

Fig a: 10 000 tumoral cells injected in the brain                                                                                                      Fig a: 50 000 tumoral cells injected in the brain

F E A T U R E SNEWTON 7.0 BT100NEWTON 7.0 BT500NEWTON 7.0 FT100NEWTON 7.0 FT500
M O D A L I T Y
  • In vivo / in vitro Optical imaging platform
  • Bioluminescence detection
  • 3D Optical Tomography
  • In vivo / in vitro Optical imaging platform
  • Bioluminescence detection
  • 3D Optical Tomography
  • In vivo / in vitro Optical imaging platform
  • Bioluminescence detection
  • Fluorescence detection
  • D Optical Tomography
  • In vivo / in vitro Optical imaging platform
  • Bioluminescence detection
  • Fluorescence detection
  • 3D Optical Tomography

O P T I C S 

C a m e r a

a

L e n s

R e s o l u t i o n

aa

F i e l d  o f  V i e w

a

  • 16-bit Scientific Grade CCD Camera
  • Grade 0 / 400-900nm / 4.8 OD
  • Cooling: -90°C Delta
  • Proprietary V.070 – Fixed Focal Length Motorized lens
  • Aperture: f/0.7
  • Resolution: 2160×2160
  • Monochrome & Color imaging
  • 1 animal capacity
  • FOV Min: 12x12cm
  • FOV Max: 12x12cm
  • 3D imaging mode via 3D-IR cameras
  • 16-bit Scientific Grade CCD Camera
  • Grade 0 / 400-900nm / 4.8 OD
  • Cooling: -90°C Delta
  • Proprietary V.070 – Fixed Focal Length Motorized lens
  • Aperture: f/0.7
  • Resolution: 2160×2160
  • Monochrome & Color imaging
  • 5 animals capacity
  • FOV Min: 6x6cm
  • FOV Max: 20x20cm
  • Macro imaging mode
  • 3D imaging mode via 3D-IR cameras
  • 16-bit Scientific Grade CCD Camera
  • Grade 0 / 400-900nm / 4.8 OD
  • Cooling: -90°C Delta
  • Proprietary V.070 – Fixed Focal Length Motorized lens
  • Aperture: f/0.7
  • Resolution: 2160×2160
  • Monochrome & Color imaging
  • 1 animal capacity
  • FOV Min: 6x6cm
  • FOV Max: 20x20cm
  • Macro imaging mode
  • 3D imaging mode via 3D-IR cameras
  • 16-bit Scientific Grade CCD Camera
  • Grade 0 / 400-900nm / 4.8 OD
  • Cooling: -90°C Delta
  • Proprietary V.070 – Fixed Focal Length Motorized lens
  • Aperture: f/0.74
  • Resolution: 2160×2160
  • Monochrome & Color imaging
  • 5 animals capacity
  • FOV Min: 6x6cm
  • FOV Max: 20x20cm
  • Macro imaging mode
  • 3D imaging mode via 3D-IR cameras

E X C I T A T I O N

W h i t e – L i g h t

F l u o r e s c e n c e

  • Dual EPI-White light LED panels
  • Upgradeable to Fluorescence
  • Dual EPI-White light LED panels
  • Upgradeable to Fluorescence
  • Dual EPI-White light LED panels
  • 8 Fluorescent channels included 420 / 480 / 520 / 580 / 640 / 680 / 740 / 780nm
  • Dual EPI-White light LED panels
  • 8 Fluorescent channels included 420 / 480 / 520 / 580 / 640 / 680 / 740 / 780nm

E M I S S I O N

F i l t e r  W h e e l

E m m i s s i o n
F i l t e r s

  • 10-position Motorized filter wheel
  • 4 Narrow Band-pass filters included for the BLI Tomography 500 / 550 / 600 / 650nm
  • 10-position Motorized filter wheel
  • 4 Narrow Band-pass filters included for the BLI Tomography 500 / 550 / 600 / 650nm
  • 10-position Motorized filter wheel
  • 8 Narrow Band-pass filters included for the BLI Tomography 500 / 550 / 600 / 650nm
  • 10-position Motorized filter wheel
  • 8 Narrow Band-pass filters included for the BLI Tomography 500 / 550 / 600 / 650nm
D A R K R O O M
M o t o r i z a t i o n
  • Fixed Camera
  • Fixed Animal Stage
  • Z-Axis motorized camera
  • X/Y-Axis motorized Animal Stage
  • Fixed Camera
  • Fixed Animal Stage
  • Z-Axis motorized camera
  • X/Y-Axis motorized Animal Stage
A N I M A L   
H A N D L I N G
  • Heated Mouse Bed (+37°C) included
  • Animal breathers included
  • Heated Mouse Bed (+37°C) included
  • Animal breathers included
  • Heated Mouse Bed (+37°C) included
  • Animal breathers included
  • Heated Mouse Bed (+37°C) included
  • Animal breathers included