The unrivaled Newton 7.0 is ideal for chemiluminescence and bioluminescence, for in vivo and in vitro applications. It has rich features and guides you into the advanced functions in a very ergonomically designed user interface. The simple and self-understandable menu is ideal in a multi-user environment. Designed by molecular biologists, the software is very easy to use: just one click is necessary to get the optimum Western Blot image.

VILBER is a leading life science company that develops and manufactures imaging and analyzing systems for fluorescence, chemiluminescence and bioluminescence applications. Founded in 1954, Vilber is a leader in the molecular imaging sector, and has equipped more than 20,000 laboratories worldwide. An estimated 60,000 people use their products every day in over 100 countries worldwide.

The NEWTON 7.0 is a highly sensitive optical imaging system dedicated to pre-clinical imaging of small animals in vivo. It combines the best optics and animal
handling features for optimum scientific images and results.

  • User-friendly instrument
  • No radiation
  • Longitudinal studies / Non-invasive
  • Large throughput studies / 5 Mice capacity
FeatureBenefit
Spectra Capsules of LightThe Spectra Light Capsules are powerful fluorescence exciters with focused light for uniform illumination and enhanced power. Equipped with primary and secondary optics, the Light Capsules are categorized as Laser Class II due to their intense power.

Benefit from an excellent signal background ratio for imaging and detect the lowest signal concentration.

Full Spectrum Tunability8 Excitation sources are available to cover the complete spectrum from Blue to infra-red.

Only 40nm separates each wavelengths and each Capsule produces a monochromatic light of a different wavelength with a very narrow-band illumination.

This reduces the cross stimulation and increases the sensitivity of your images.

From Macro-imaging to large throughput studiesThanks to an innovative darkroom architecture, position your samples or animals at various levels depending on the sample size or the number of
animals to be imaged.The closer to the camera, the higher level of sensitivity!
10 Megapixels image resolutionHigh Resolution CCD camera
90 C via 4 Peltier Thermoelectric coolerDeep CCD Cooling Best Signal to Noise Ratio
f/0.7 Proprietary lensWidest lens aperture Highly sensitive
4.8 Optical DensityExtremely linear over its wide dynamics

Fluorescence is the phenomenon by which certain molecules under illumination absorb light of a specific wavelength and normally react by emitting light of a comparatively longer wavelength.

This phenomenon relies on the production of excited single states. An electron in the excited orbital is paired with a second electron (of opposite spin) in the
ground-state orbital.

A photon is emitted as a result, causing a rapid return to the ground state.

                                             

Wavelengths are known to determine the degree of absorption and scattering in tissue and therefore the penetration depth in tissues.

Using near-infrared light permits detecting tissue structures deeper under the surface than using visible light. In particular, NIR wavelengths (between 650 and
900 nm) were observed to travel more efficiently through tissues than ones in the visible light spectrum.

In addition to light absorption and scattering in living tissues, tissue autofluorescence can also noticeably limit contrast in fluorescence optical imaging.

NIR light solves this problem by reducing the autofluorescence background of intrinsic fluorophores and tissue depths of several centimeters can be achieved.

                                                 
                             Lung Cancer                                                                                                                              Xenograft Tumor

Bioluminescence imaging is most commonly used for in vivo optical imaging and refers to the light that is generated by a chemical reaction between the substrate, luciferin, and oxygen, in which luciferase acts as the enzyme to accelerate the reaction.

                             

Simultaneous monitoring of red and green fluorescent signals by dual-color in vivo imaging
Signals can be overlaid so that several reporters can be visualized simultaneously.

Images that were acquired during different sessions can be grouped together to form a sequence. For example, a time series could be constructed from images acquired on different days following an experimental treatment.

The software allows to collect and compare the data throughout the experimental treatment.

 
           Day 1                               Day 5                             Day 10                              Day 15                           Day 20                          Day 25                             Day 30                            Day 35

NiraWave M is a micellar formulation of the clinically proven near infrared fluorescence dye indocyanine green (ICG). Due to the unique formulation, NiraWave M exhibits a stronger fluorescence, higher aqueous stability and prolonged blood circulation time. It is optimized for fluorescence angiography, particularly for the visualization of the microcirculation.

Highlight vascular structures and benefit from the strong and long lasting blood vessel contrast allowing studies of the microcirculation

Benefit from the deep tissue penetration: The high aqueous stability prevents rapid fluorescence decay of the ICG molecule.

                                     

NiraWave Rocker is a nanoparticulate near near-infrared (NIR) fluorescence imaging agent optimized for visualization of vascular leakage in inflamed tissues and tumors. Besides allowing for studies of the enhanced permeability and retention (EPR) effect, the agent also accumulates in certain cell types via the rocker rocker-switch mechanism.

Highlight inflamed tissues and tumors and benefit from the deep tissue penetration.

Apply NiraWave Rocker to:

-Detect areas of increased vascular permeability, e.g. certain types of tumors

-Study inflammatory processes such as in rheumatic diseases.

-Monitor therapeutic response

Images that were acquired during different sessions can be grouped together to form a sequence. For example, a time series could be constructed from images acquired on different days following an experimental treatment.

The software allows to collect and compare the data throughout the experimental treatment.

3D Dynamic Scan

Live 3D representation of the signal to source the localization as well as to detect signal concentration.

Fluorescence biomarker imaging using VIS probes provides insights of inflammatory responses in a preclinical model.

Inflammation were assessed using in vivo non invasive molecular imaging.


Rheumatic arthritis visualization

The biodistribution of DOX:CS/CMCS-NGs was investigated in rats (Sprague–Dawley, 220–250 g) and the fluorescent signal of DOX was detected by the small animal in vivo imaging instrument


In vivo noninvasive fluorescence imaging
Biodistribution study DOX distribution in rats

The major organs were dissected at 10 h after oral administration and observed by ex vivo imaging.

The results of quantitative experiment showed that all organs in DOX:CS/CMCS-NGs group exhibited significant higher DOX level than DOX:CS/CMCS-NGs with Ca2+ group.


Ex vivo fluorescence imaging of organs
at 10 h post-oral-administration

Qualitative detection of tissue distribution of drug loaded fluorescent nano particles to detect the penetration into turbot fish.

After 36 h, heart and liver were extracted to be visualized under macro-imaging in order to observe the distribution of FITC-BSA in organs.

                           
Distribution of FITC-BSA in turbot fish at different time                                                                            In vivo fluorescence imaging of FITC-BSA distribution in organs at 36 h

Bacterial passage through the mouse intestine determines the colonization dynamics of intestinal pathogens such as ETEC.

Streptomycin-treated BALB/c mice were intraperitoneally administered with 200 μL (15 ng/μL) of luciferin and gastrically gavaged with 1 × 108 CFU ETEC FMU073332 harboring pRMkluc and E. coli K-12 harboring pRMkluc.

After inoculation, the bioluminescent signals displaced toward the small intestine. 48 hours after gastric inoculation, the bioluminescent signals indicated bacterial passage through the mouse intestine. The bacterial bioluminescent signals remained in the mouse intestine after the 120 h postinoculation.


Bioluminescence imaging of bacterial passage
through the mouse gastrointestinal tract.

After 120 h of E. coli infection, mouse gastrointestinal tracts were extracted to perform ex vivo imaging. Intestinal tract dissection comprised the esophagus to the rectum.

The bioluminescent signals emitted by ETEC FMU073332 harboring pRMkluc were located in the proximal mouse ileum approximately 6 cm from the cecum, whereas the control signals were identified in the cecum and in the proximal colon.


Bioluminescence imaging of bacterial passage
through the mouse gastrointestinal tract.

In vivo tracking of bacteria allows to understand the role of ETEC during bacterial infection.

BALB/c mice were intraperitoneally inoculated with E. coli K-12 harboring pRMkluc or E. coli K-12 harboring pBR322 (incubated with luciferin).

After 1 h of infection, bioluminescent signal emission from the animals was captured.

Mice infected with E. coli K-12 harboring pBR322 did not exhibit bioluminescent emission (Fig.b) However, mice infected with E. coli harboring pRMkluc emitted bioluminescent signals in the mouse inoculation zone (Fig.a).


in vivo bioluminescence emission of E. coli K-12

The purpose of this experiment is to follow the spread of the drug into the animal body.

Fluorescent label offers a viable screening option


Fluorescence imaging of Cy5.5 expression in the living mouse after injection of a fluorescent tracer.

Injection of 10,000 (fig.a ) and 50,000 fig.b ) tumoral cells expressing luciferase into the brain of the mice .

After a 6 weeks tumor grow , it is possible to visualize the linearity between mice with 10 000 or 50 injected cells , giving the possibility to follow the tumor growth .

                                                                                   
Fig a: 10 000 tumoral cells injected in the brain                                                                                                      Fig a: 50 000 tumoral cells injected in the brain

SystemNewton Mini 7.0Newton 7.0 FT400
Optics
Camera16-bit Scientific Grade CCD
CCD Cooling-90 C
Image Resolution10 Megapixels
LensFixed Focal Length f/0.7 Motorized
Excitation and Emission
Epi-FluorescenceOptional individual channels8 Channels included
Emission filtersOptional narrow bandpass filters9 Filters included
Filter Wheel7-position Motorized10-position Motorized
Darkroom Architecture
CameraFixedZ-Axis Motorized
StageHeight Adjustable (manually)X/Y-Axis Motorized
Animal Capacity3 mice / 1 rat5 mice / 3 rats
Animal Accessories
Heated bed+37°C thermoregulated bed included
Animal breathers3 or 5 animal breathers with nose cones included
WAGWaste Gas Scavenger included
AnesthesiaBiosthesia compatible (optional)

Supported by prestigious publications

Peng Xue , Mengmeng Hou, Lihong Sun et al.
Calcium carbonate packaging magnetic polydopamine nanoparticles loaded with indocyanine green for near infrared […] therapy
Acta Biomaterialia 10.1016/j.actbio.2018.09.045

Xiu Jun Fu, Yun Qing Zhu, Yin Bo Peng et al.
Enzyme activated photodynamic therapy for methicillin resistant Staphylococcus aureus infection both in vitro and in vivo
Journal of Photochemistry and Photobiology B: Biology 10.1016/j.jphotobiol.2014.04.016

Peng, Yinbo , Chenlu Song, Chuanfeng Yang et al.
Low molecular weight chitosan coated silver nanoparticles are effective for the treatment of MRSA infected wounds
International Journal of Nanomedicine 10.2147/IJN.S122357

Gerardo E. Rodea , Francisco X. Montiel Infante , Ariadnna Cruz Córdova et al.
Tracking Bioluminescent ETEC during in vivo BALB/c Mouse Colonization
Front Cell Infect Microbiol. 10.3389/fcimb.2017.00187

Huizi Keiko Li, Yukie Morokoshi , Kotaro Nagatsu et al.
Locoregional therapy with α‐emitting trastuzumab against peritoneal metastasis of human epidermal growth factor receptor 2‐positive gastric cancer in mice
Cancer Science 10.1111/cas.13282

Ming Kong, Lin Hou, Juan Wang et al.
Enhanced transdermal lymphatic drug delivery of hyaluronic acid modified transfersomes for tumor metastasis therapy
Chemical Communications 10.1039/c4cc08746a

Chao Feng, Guohui Sun, Zhiguo Wang et al.
Transport mechanism of doxorubicin loaded chitosan based nanogels across intestinal epithelium
Europ . J. of Pharmaceutics and Biopharmaceutics 10.1016/j.ejpb.2013.11.007

Qian Li, Lihong Sun, Mengmeng Hou et al.
A Phase change Material Packaged within Hollow Copper Sulfide Nanoparticles Carrying Doxorubicin and Chlorin e6 for Fluorescence guided Trimodal Therapy
of Cancer
ACS Appl. Mater. Interfaces, 10.1021/acsami.8b19667

Peng Xue , Mengmeng Hou, Lihong Sun et al.
Calcium carbonate packaging magnetic polydopamine nanoparticles loaded with indocyanine green for near infrared induced photothermal/photodynamic
therapy
Acta Biomaterialia 10.1016/j.actbio.2018.09.045