CO2 and Tri-gas incubators are essential items of lab equipment for any researcher performing cell culture work. A good incubator create a contamination-free environment for cells while maintaining temperature, humidity and O2/CO2 levels. Further all modern incubators must be able to quickly restore conditions when an incubator door is opened. The smart D180-P line of incubators from RWD can both maintain and quickly restore internal conditions but do essential task this at a fraction of the price of comparable systems.
RWD incubators offer users accurate control of temperature, humidity and gas concentrations via an easy to program intelligent touch screen system. These systems also offer users an automatic data record for 7 days including a straightforward and easy to understand curve display. The D180-P uses a 6-sided air jacket system and heated inner door to uniformly control internal temperatures.
Moreover, ultra-reliable RWD incubators feature a built-in HEPA air filtration system that continuously filters the entire chamber volume. The polished stainless-steel interior diminishes the chances of contamination growth within (full copper interior also available for added protection). Additionally, an on-demand 140°C high-temperature sterilization cycle eliminates contamination from all internal surfaces simplifying cleaning procedures.
Easily set or change set-points on a 7-inch touch screen menu. The intelligent touch screen interface is designed to provide complete data visibility to monitor all incubator interactions, featuring door-mounted position for easy access, on-screen menu prompts, error and usage logs, data logging, performance trend graphing.
Fan-assisted air circulation for uniformity and rapid recovery
Fan-assisted technology allows for advanced uniformity and recovery. The internal airflow pattern is precisely designed for excellent distribution of all critical environmental conditions (temperature, humidity and gas exchange). Fan-assisted circulation minimizes variation between cultures, while slowing desiccation. No matter where your cultures are located in the incubator they will be under uniform conditions.
in-chamber CO2 measuring technologies
CO2 sensors are placed within the chamber ensuring highly accurate control. The RWD systems use the most precise sensor technology which function perfectly regardless of what conditions are set for the chamber. The infrared (IR) sensor technology used in the D180-P allows for accurate monitoring even when temperature and humidity levels are less predictable.
High temperature sterilization for easy cleaning
With the simple touch of a button (via the touch-screen) decontaminate your incubator using an on-demand 140° C dry heat sterilization cycle and eliminate the need for separate autoclaving and re-assembly of components. This safe and effective high-temperature sterilization cycle is proven to effectively eliminate bacteria, mold, yeast, mycoplasma and even resistant spores, simplifying cleaning protocols and protecting cultures and personnel.
Optional O2 control
Many cell types thrive best in CO2 incubators with reduced oxygen. A case could made that most mammalian cell lines should be cultured under physiologically relevant conditions (see an article on this topic here). Culturing cells at lower oxygen concentration will better simulate physiological conditions, resulting in cell behaviors that are more predictive of the in vivo environment. With variable oxygen control incubators one can simulate physiologically relevant or hypoxic environments for various cell research applications from 1–21%.
CondoCell – is a robust isolation box for any sensitive cell culture in T-flasks, petri dishes or multi-well plates. It fully captures the environment of any incubator or hypoxia workstation making non-stop, uninterrupted culture possible.
PhO2x Box – Adds and isolated environment into any incubator. Grants researchers’ full control of O2 and CO2 while utilizing the temperature and humidity of the incubator.
What is the recommended cleaning/disinfecting procedure for the D180-P?
The recommended disinfecting agent for use with the incubator is a solution of 70% isopropanol or ethanol and 30% sterile distilled water. It is also recommended to do high temperature disinfection every 6 months to maintain full sterility.
What tasks should be done daily for good maintenance
Check the CO₂/N₂ gas tank levels and ensuring that the pressure is not low. Replace it with a new tank if necessary.
What tasks should be done weekly for good maintenance
Check the water level of the pan and ensure that there is enough water in the pan for humidity. Refill with sterile distilled if needed. Do not use tap water.
Follow your own procedure of disinfecting the interior components and exterior surfaces.
Are gloves necessary to operate the D180-P?
Wearing gloves represents good laboratory practice with all lab bench work, but there is no requirement for the user to wear them.
What gases are required to operate the D180-P?
One gas is needed to operate the standard unit is laboratory grade quality 100% CO2 is needed.
Where should the Incubator not be installed in a lab?
Avoid areas that are close to air ducts, windows, and doors as fast moving air currents create temperature fluctuations which can affect the temperature controls of the equipment. Also never install in direct sunlight, or near devices that produce excess heat such as autoclaves, space heaters, ovens, and others. Lastly, avoid placing the equipment near vibrating devices such as centrifuges and vortex mixers. The vibration greatly affects the growth of cells which consequently affects your research results.