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Small Vessel Wire Myograph Systems2018-03-27T21:38:55+00:00

Small Vessel Wire Myograph Systems

The system shows a unique modularity with features as follows:

  • Rapid extension to 1-4 channels,
  • Removable organ bath (with 2, 5, 10, 20 mL capacity),
  • Isometric measuring sensor,
  • Two sets of vessel holders, depending on the diameter of the vessel being examined (Ø60 µm – Ø3 mm),
  • Gas controller, with a freely positioned diffuser tip,
  • Continuous temperature control as per request,
  • Covering lid against evaporation and heat loss,
  • Locking of the vessel holder to facilitate positioning of the organ,
  • The measuring unit (organ bath, manipulator, force transducer) can be unlocked and removed from the base plate,
  • Field-stimulation electrodes (as per request),
  • Temperature stabilization: circulating water bath thermostat.
MDE GmbH - Small Vessel Wire Myographic Systems
System Catalogue

The System Consists of Five Main Units:

  1. Organ bath (flow-through/stationary) with buffer in-and outflow stubs (gravitational or pumped flow) gas controller with freely positionable diffuser tip, and field-stimulating electrodes (as per request). Vessel holders: tungsten wires or steel prods (depending on vessel lumen); vessel holder locking for positioning of the organ
  2. In- and outlet stub of the thermostated circulating water bath (20 l, 15 l/min flow rate)
  3. Precision manipulator (0.25 mm/turn)
  4. Isometric sensor
  5. Base plate with lock/unlock lever for the measuring unit
MDE GmbH - Small Vessel Wire Myograph Systems - Scheme of the System

Organ Bath, Measuring Unit

The organ bath is manufactured in four different volumes (2, 5, 10, 20 ml). The standard volume is 5 ml unless requested otherwise. Each volume is available for later order, and can be replaced in the measuring unit.

The tubes carrying gas and solution for maintaining optimal physiological environment are connected to the measuring unit through connectors fixed in the base plate. Removable design of the measuring unit greatly facilitates placement of the preparation in the vessel holder because the unit can be put on the stage of a microscope.

Removing the Measuring Unit

The organ bath, the tension manipulator and the sensor are built into one unit which can be removed, for the sake of easy handling, in the following way:

STEP 1: Turn the lever locking the unit to the base plate and tube connector in 90º angle

MDE GmbH - Small Vessel Wire Myograph Systems - Unit Locking

STEP 2: Slide out the unit along the guide rail of the base plate

MDE GmbH - Small Vessel Wire Myograph Systems - Unit Sliding
MDE GmbH - Small Vessel Wire Myograph Systems - Measuring Unit and Baseplate

Vessel Holders, Precision Setting of Gas Supply

The tungsten wire vessel holder type ISO-12-21 is recommended for vessel preparations with an inner diameter between 0.05 mm and 0.5 mm, and length up to 3.5 mm.The choice of vessel holders is determined by the inner diameter and length of the preparation. The decision finally depends on the professional competence of the person performing the experiment. The vessel holder can be interchanged in the sleeves, within and between types.

The steel prod vessel holder type ISO-12-22 can be used also for preparations with an inner diameter between 0.8 mm and 3 mm, and length over 3.5 mm. This technical solution enables the continuous examination of larger vessels in a small volume of buffer.

Gas Controller and Diffuser

For proper working with the preparation, it is vital to have the necessary amount and adequate quality of carbogen gas evenly distributed in the solution.
To ensure the sufficient dispersion, a bubbler and fine-tuner device has been developed. The gas coming through the pressure reducer on the gas cylinder is kept in a gas bag. From there, the gas flows in a sufficiently thick-walled tube through a cylindrical clamp and is applied into the solution via a tube-tip gas diffuser.

Calibration

Calibration is done by means of an independent unit which can be connected to the system.

Components include:

  • Holder with the pulley with bearing
  • Thread with hook
  • 1g certified weight

Calibration process:

  • Remove the precision manipulator and replace it with the pulley holder
  • Lock the isometric sensor with the lever. Hitch the thread to the stub on the vessel holder sleeve of the sensor, then lead it over the pulley
  • Attach the 1g calibration weight to the hook of the thread
  • Unlock the sensor by turning the lever back in 90º

Accessories Required for Operation

The analog amplifiers, the computer and the software constitute an autonomous, integrated unit in the workstation. This solution allows fast configuration, training and promotes concentration on the experimental work.

Recommended Software/Hardware for Recording

The following hardware-software systems are recommended to graphically display, store and analyze the measured physiological parameters:

  • SPEL ISOSYS (MDE GmbH),
  • SPEL KYMOGRAPH (MDE GmbH),
  • LabChart (ADInstruments),
  • AcqKnowledge (BIOPAC),
  • LabScribe (iWorx).

Click the product code below for details

NOTE: This system is also available in two- (IS-05-02) or four-channel (IS-05-04) models. Please contact us for further details.

CODE NAME
Base with taps and gas adjuster (capacity for two or four units)
10ml organ bath with force transducer and one-plane manipulator (250µm)
 EXP-SG-1 DC bridge amplifier
Micro vessel holder clamps
100µm wolfram filament (L=3m)
OPTIONAL
ISO-12-61 50µm wolfram filament (L=3m)
ISO-12-63 200µm wolfram filament (L=3m)
ISO-12-64 300µm wolfram filament (L=3m)
Precision peristaltic pump
EXP-TH-1 One-channel amplifier for measuring temperature with sensor
EXP-ST-01 One-channel non-isolated stimulator with MCU controlling unit
Field stimulating electrodes for organ bath (1 pair)
CWB-02 Circulating water bath (20 litres, 20 l/min delivery speed, ±0,1ºC)
SOFT-02 MDE – SPEL ISOSYS Monitoring, measuring and analysing software (16ch) system with accessories*
SOFT-03 MDE – SPEL KYMOGRAPH Monitoring, measuring and analysing software (8ch) system with accessories*
ADI-PL ADI – Power Lab with LabChart PRO software*
iWorx-LBS iWorx – LabScribe software*
Biopac-Acq BIOPAC – AcqKnowledge*
*You only need to purchase one (1) of the data acquisition systems above

References

YEAR AUTHOR TITLE SOURCE
2013 Broskova Z, Sotnikova R, Nedelcevova J, Bagi Z. Effect of a novel stobadine derivative on isolated rat arteries Interdiscip Toxicol. 2013 Jun;6(2):63-6. doi: 10.2478/intox-2013-0011.
2012 Sotnikova R, Bacova B, Vlkovicova J, Navarova J, Tribulova N. Sex differences in endothelial function of aged hypertriglyceridemic rats – effect of atorvastatin treatment Interdiscip Toxicol. 2012 September; 5(3): 155–158.
2011 Vata LG, Plamadeala P, Foia LG, Mitu F, Gorgan L, Vata A, Dumitriu IL, Gurzu B. Obesity independent effects of high fat diet on pulmonary arteries reactivity Acta Endocrinologica Oct-Dec2011, Vol. 7 Issue 4, p451
2010 Vata LG, Dumitriu IL, Gurzu M, Slatineanu S, Vata A, Gurzu B. Ghrelin effects on local renin angiotensin from pulmonary vessels Acta Endo (Buc) 2010, 6 (3): 295-304 doi: 10.4183/aeb.2010.295
2010 Camelia, Mihai Ana; Maria, Dinca; Luciana, Dumitriu Irina; Beatrice, Gurzu Mihaela; Mihaela, Slătineanu Simona; Georgeta, Foia Liliana; Gina, Vata Luminita; Elena, Cojocaru; Gheorghe, Petrescu Apelin involvement on angiotensin II – induced venoconstriction Fiziologia – Physiology, 2010, Vol. 20 Issue 3, p8
2003 Rodríguez-Sinovas A, Bis J, Anivarro I, de la Torre J, Bayés-Genís A, Cinca J. Coronary smooth muscle reactivity to muscarinic stimulation after ischemia-reperfusion in porcine myocardial infarction J Appl Physiol (1985). 2003 Jul;95(1):81-8. Epub 2003 Mar 7.
2002 Haj-Yehia A, Nassar T, Kadery B, Lotan C, Da’as N, Kleinman Y. Effects of the SOD mimic nitroxide 3-carbamoyl-PROXYL on oxidative stress markers and endothelial dysfunction in streptozotocin-induced diabetic rats Exp Clin Cardiol. 2002 Fall;7(2-3):85-92.
MDE GmbH - Small Vessel Wire Myograph Systems - Catalogue

click image to download catalogue (PDF)