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Modular Tissue Bath Systems2018-11-30T12:25:14+00:00

Modular Tissue Bath Systems

Solutions for smooth and striated muscle investigation

System features:

  • Easy upgrade to 2, 4, 8 channels
  • Replaceable tissue baths (5, 10, 20 ml calibrated volume)
  • Special vertical tissue baths with small volume (1, 2 ml calibrated volume)
  • Tissue holders with built in electrodes for point or field stimulation and with gas bubbler
  • Holders for vessel ring with suitable size (from Ø0,8mm) for built in stimulating electrodes
  • Gas chamber and gas flow controller
  • One-plane self-adjusting manipulator with sensor clamp (300µm)
  • Buffer reservoire with volume of 0,4 litre and 1,5 litre according to choice per channel
  • Vibration free stand
  • External thermostation (20 litre, 15 litre/min circulation speed, 0,1ºC accuracy
  • Force/displacement sensors (SEN-03-3X from 10g up to 500g)
  • Four channel bridge amplifier (EXP-SG-4)
MDE GmbH - Isolated Muscle Tissue Systems - Four-Channel Modular Tissue Bath System
System Catalogue

Theoretical Setup

In order to keep basic physiological parameters in the system stable, a coordinated operation is required. The associated flow chart shows the operation of the system. To keep the tissue sample alive an optimal buffer amount, flow and temperature is necessary.  This is achieved by control of buffer flow to the chambers, gas bubbling (Carbogen), and keeping temperature stable.

 
MDE GmbH - Isolated Muscle Tissue Systems - Scheme Of The Four-Channel System
 
 
 
 

Main Components

[A] Organ chamber

[B] Buffer reservoir

[C] Circulating water bath

[D] Carbogen cylinder

 
 

The tissue bath [A] is filled with buffer from the reservoir [B], operated by a two-way tap. The tissue bath has two outlets. The upper outlet is an overflow to keep the calibrated volume. The lower outlet is for emptying the tissue chamber during operation or during cleaning. Calibration of the tissue chamber is based on fluid displacement in the tissue chamber by the tissue holder with 1% accuracy. (eg. in case of 10ml organ chamber: inner diameter of the organ chamber = Ø 18mm, tissue holder diameter = Ø 17,5mm).

The circulating water bath [C] maintains a continuous and stable temperature. The unit keeps the flow of water in a closed circuit providing stable temperature in the water jacket of the the tissue chamber and the buffer reservoir. The Carbogen cylinder [D] supplies the system with Carbogen gas. The fluid-filled reservoirs and tissue chambers are supplied with gas from this chamber through an adjuster and a fine-pore bubbler.

 
 
 
 
 

Buffer Reservoire, Tissue Bath, Sensor Holder, Gas Supplier with Adjuster

 

Buffer reservoire, tissue bath, sensor holder and gas supplier with adjuster are the main components of one complete unit. These are assembled on the stand as one unit, then the tubing connections is done. This structure makes the replacement of any parts easy in case of damage. Moreover, malfunction of any part in the unit does not influence the operation of other units in the system, which is a further advantage.

 
 
 
 
 
 
 

Parts of the Holder

The reservoires and the tissue bathes are supplied with gas through a gas chamber. Each reservoire and tissue bath is equipped with gas adjuster and bubbler. Bubblers are immersed into the buffer and maintaine the dissolved gas concentration. For the proper function of the tissue sample a suitable gas content in the buffer is absolutely necessary. To make a large bubble surface for effective gas exchange a gas bubbler with adjuster was developed. Gas, coming from the cylinder through a reductor, is collected in a gas regulator then is driven into the bubbler head and to the buffer through a tube with suitable wall thickness.

 
 
 
 
 

The Tissue Holder and Tissue Bath Volume

The tissue bath unit comprises of two holders on the vertical bar:

  • The upper one which cannot be moved
  • The lower one which holds the tissue bath and the two-way tap for filling and can be moved

The tissue holders has four functions:

  • Setting of tissue bath volume by volume displacement with 0.1% accuracy
  • Providing gas into the buffer by a built in gas bubbler
  • Providing electrical stimulation of the tissue sample by built in stimulating electrodes
  • Providing a centralized suspension of the tissue sample

The bottom shape of the tissue holders enable effective buffer change/wash out and prevents bubbles from sticking on the surface.

NOTE: Vessel ring holders (from Ø0,8mm) are also available for built-in stimulating electrodes.

 
 
 
 
 

Tissue Application, Pre-Tension

During the development of the tissue bath system our goal was to get the tissue sample onto the tissue holder with a possible less stress and immerse as fast as possible into the oxigenated buffer. The handy solutions serve for the harmonized functionality.

 

STEP 1: The tissue holder has to be „pulled out” by lowering the tissue bath holder after loosening its fixing bolt in the rear. Then the holder is fixed again with the bolt. One end of the tissue strip is fixed onto transducer arm, the other end is fixed onto the hook on the tissue holder.

STEP 2: The holder with tissue bath is slid upwords after loosening the fixing bolt until the tissue holder with the sample on it fully immersed into the carbogenized buffer.

STEP 3: The excess of buffer drained through the overflow providing the previously set calibrated volume in the chamber. Then the bolt on the rear is fixed again.

 
MDE GmbH - Isolated Muscle Tissue Systems - Tissue Application
 
 
 
 

Small Volume Tissue Bathes (SD1-v0, SD2-v0)

A complementary SD tissue bath with 1ml or 2ml volume is available. These bathes are also equipped with bubbler, tissue holder, etc. like standard bathes.

 

STEP 1: Lower the tissue bath holder after loosening its fixing bolt in the rear. Remove the tissue holder and replace it with the SD bath then fix it with a screw.

STEP 2: Pull out the tissue holder of SD bath. Place the tissue sample on it.

STEP 3: Lift the tissue bath holder until the SD bath immersed into the buffer. The SD bath will be filled with the buffer. Following this lower the tissue bath again until the SD just above the buffer level in the tissue bath. Fix the organ bath holder with the bolt on the rear.

 
 
 
 
 

Data Acquisition & Analysis

For recording and analysis of measured parameters we offer the following software/hardware systems:

  • SPEL ISOSYS (MDE GmbH)
  • SPEL KYMOGRAPH (MDE GmbH)
  • LabChart (ADInstruments)
 

Accessories Required For Operation:

 

Click the product code below for details

CODE QUANTITY NAME
1 Stand with taps (4 pcs) and one-plane stretcher manipulators (4 pcs)
4 Organ chambers with holders*
4 Buffer reservoires (0.4 l/pc)
4 Teflon tissue holders with gas vaporizer and stimulating electrode**
OPTIONAL
EXP-SG-4 1 Four- channel DC bridge amplifier
SEN-03-3X 4 Force/displacement transducer (select the size of the sensor)
EXP-ST-04 1 Four-channel non-isolated stimulator with MCU controlling unit
CWB-02 1 Circulating water bath (20 litres, 20 l/min delivery speed, ±0,1°C)
ISO-08-5X 1 Vessel ring holders*** (10 pairs/pack)
SOFT-02 1 MDE – SPEL ISOSYS Monitoring, measuring and analysing software (16ch) system with accessories****
SOFT-03 1 MDE – SPEL KYOMGRAPH Monitoring, measuring and analysing software (8ch) system with accessories****
ADI-PL 1 ADI Power Lab with LabChart PRO software****
iWorx-LBS 1 iWorx – LabScribe software****
Biopac-Acq 1 BIOPAC – AcqKnowledge****
*Choose organ chamber volume: 5 ml, 10 ml or 20 ml.
**Choose the type of stimulation: no, point, ring, zig-zag or other.
***Choose the type from ISO-08-5X datasheet.
****You only need to purchase one (1) of the data acquisition systems above
CODE QUANTITY NAME
2 Stand with taps (4 pcs) and one-plane stretcher manipulators (4 pcs)
8 Organ chambers with holders*
8 Buffer reservoires (0.4 l/pc)
8 Teflon tissue holders with gas vaporizer and stimulating electrode**
OPTIONAL
EXP-SG-4 2 Four- channel DC bridge amplifier
SEN-03-3X 8 Force/displacement transducer (select the size of the sensor)
EXP-ST-04 2 Four-channel non-isolated stimulator with MCU controlling unit
CWB-02 1 Circulating water bath (20 litres, 20 l/min delivery speed, ±0,1°C)
ISO-08-5X 1 Vessel ring holders*** (10 pairs/pack)
SOFT-02 1 MDE – SPEL ISOSYS Monitoring, measuring and analysing software (16ch) system with accessories****
SOFT-03 1 MDE – SPEL KYOMGRAPH Monitoring, measuring and analysing software (8ch) system with accessories****
ADI-PL 1 ADI Power Lab with LabChart PRO software****
iWorx-LBS 1 iWorx – LabScribe software****
Biopac-Acq 1 BIOPAC – AcqKnowledge****
*Choose organ chamber volume: 5 ml, 10 ml or 20 ml.
**Choose the type of stimulation: no, point, ring, zig-zag or other.
***Choose the type from ISO-08-5X datasheet.
****You only need to purchase one (1) of the data acquisition systems above

References

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2012 Appiah I, Nikolic-Kokic A, Orescanin-Dusic Z, Radojicic R, Milovanovic S, Spasic M, Blagojevic D. Reversible oxidation of myometrial voltage-gated potassium channels with hydrogen peroxide Oxid Med Cell Longev. 2012;2012:105820. doi: 10.1155/2012/105820. Epub 2012 Oct 24.
2012 Kitic D, Brankovic S, Radenkovic M, Savikin K, Zdunic G, Kocic B, Velickovic-Radovanovic R. Hypotensive, vasorelaxant and cardiodepressant activities of the ethanol extract of Sideritis raeseri spp. raeseri Boiss & Heldr J Physiol Pharmacol. 2012 Oct;63(5):531-5.
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2011 Brankovic S, Kitic D, Radenkovic M, Veljkovic S, Jankovic T, Savikin K, Zdunic G. Spasmolytic activity of the ethanol extract of Sideritis raeseri spp. raeseri Boiss. & Heldr. on the isolated rat ileum contractions J Med Food. 2011 May;14(5):495-8. doi: 10.1089/jmf.2010.0036. Epub 2011 Apr 11.
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2010 Klukovits A, Tekes K, Gündüz Cinar O, Benyhe S, Borsodi A, Deák BH, Hajagos-Tóth J, Verli J, Falkay G, Gáspár R. Nociceptin inhibits uterine contractions in term-pregnant rats by signaling through multiple pathways Biol Reprod. 2010 Jul;83(1):36-41. doi: 10.1095/biolreprod.109.082222. Epub 2010 Mar 17.
2009 Minorics R, Gáspár R, Gál A, Klukovits A, Falkay G. Progesterone decreases the relaxing effect of the beta3-adrenergic receptor agonist BRL 37344 in the pregnant rat myometrium Reproduction. 2009 Aug;138(2):383-90. doi: 10.1530/REP-09-0116. Epub 2009 May 21.
2009 Sprossmann F, Pankert P, Sausbier U, Wirth A, Zhou XB, Madlung J, Zhao H, Bucurenciu I, Jakob A, Lamkemeyer T, Neuhuber W, Offermanns S, Shipston MJ, Korth M, Nordheim A, Ruth P, Sausbier M. Inducible knockout mutagenesis reveals compensatory mechanisms elicited by constitutive BK channel deficiency in overactive murine bladder FEBS J. 2009 Mar;276(6):1680-97. doi: 10.1111/j.1742-4658.2009.06900.x. Epub 2008 Feb 12.
2009 Appiah I, Milovanovic S, Radojicic R, Nikolic-Kokic A, Orescanin-Dusic Z, Slavic M, Trbojevic S, Skrbic R, Spasic MB, Blagojevic D. Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus Br J Pharmacol. 2009 Dec;158(8):1932-41. doi: 10.1111/j.1476-5381.2009.00490.x.
2009 Orescanin-Dusić Z, Milovanović S, Radojicić R, Nikolić-Kokić A, Appiah I, Slavić M, Cutura N, Trbojević S, Spasić M, Blagojević D. Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated Gen Physiol Biophys. 2009;28 Spec No:143-8.
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2007 Csonka D, Zupkó I, Minorics R, Márki A, Csík G, Falkay G. The effects of alpha-methyldopa on myometrial noradrenaline release and myometrial contractility in rat Acta Obstet Gynecol Scand. 2007;86(8):986-94.
2007 Brugós L, Gesztelyi R, Zsuga J, Cseppento A, Benko I, Galajda Z, Deák G, Sipka S, Roszer T, Kovács P, Szilasi M, Edes I, Szentmiklósi AJ. Modulation of adenosine-induced responses in the guinea-pig trachea during long-term caffeine treatment: possible role of epithelium J Pharmacol Sci. 2007 Nov;105(3):279-90.
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2005 Gáspár R, Ducza E, Mihályi A, Márki A, Kolarovszki-Sipiczki Z, Páldy E, Benyhe S, Borsodi A, Földesi I, Falkay G. Pregnancy-induced decrease in the relaxant effect of terbutaline in the late-pregnant rat myometrium: role of G-protein activation and progesterone Reproduction. 2005 Jul;130(1):113-22.
2004 Klukovits A, Gaspar R, Santha P, Jancso G, Falkay G. Role of capsaicin-sensitive nerve fibers in uterine contractility in the rat Biol Reprod. 2004 Jan;70(1):184-90. Epub 2003 Oct 1.
2003 Gesztelyi R, Zsuga J, Cseppentõ A, Bajza A, Varga A, Szabó JZ, Szentmiklósi AJ. Special sensitization pattern in adenosine-induced myocardial responses after thyroxine-treatment J Pharmacol Sci. 2003 Apr;91(4):295-304
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MDE GmbH - Modular Tissue Bath Systems Brochure

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