The iNSiGHT DXA system is a state-of-the-art in vivo Dual Energy X-Ray Absorptiometry (DXA/DEXA) technology designed for preclinical research. It provides a comprehensive solution for evaluating a wide range of metabolic disorders, such as osteoporosis, arthritis, diabetes, obesity, and musculoskeletal pathologies, including bone regeneration and muscle wasting diseases.
This system is equipped with a fully shielded X-ray cabinet, making it suitable for small animal DXA applications, with a focus on mice, rats, and animals of similar size (up to 5kg). It provides fast and efficient body composition measurements. The system’s fast scan times of 25 seconds, combined with its low-dose radiation, make it ideal for longitudinal studies.
The iNSiGHT system uses dual energy x-rays to obtain highly accurate and reproducible body composition measurements. The system assigns each pixel to one of three compartments: fat mass, non-bone/lean mass, or bone mineral content, providing measurements such as bone mineral density, bone mineral content, bone area, tissue area, fat tissue as a percentage and weight, lean tissue as a percentage and weight, and total weight in grams.
This technology is non-invasive, non-destructive, and does not require any pre-treatment of the animal, contrast, or substrate injection. The iNSiGHT system also provides specific bone length measurements that can be drawn from the 2D x-ray image.
Features & Benefits
The iNSiGHT is a Dual Energy X-Ray Absorptiometry (DXA/DEXA) system designed specifically for in vivo preclinical research applications
Easy data acquisition
No preparation steps other anesthesia required. This can be done using the integrated anesthesia system, or with injectable anesthesia if desired.
Once ready the imaging subject is placed on stage and imaging can begin, using the easy-to-use acquisition software.
Large scan area
The iNSiGHT system allows for a variety of animal models and tissue samples to be imaged, from 10g ~ 5kg. (Scan Area: 16.5 x 25.5 cm)
This allows users to image not only mice and rats, but other species such as non-human primates, fish, rabbits, etc.
25 second scan time
The iNSiGHT has the fastest scan time on the market, allowing for high-throughput imaging.
Comprehensive set of images and parameters
In a single scan (25 seconds) the x-ray attenuated image, bone mineral density map and color map are generated.
A number of parameters are calculated from these images – either from the whole animal or for user defined regions of interest (ROI):
- Bone Mineral Content (BMC) (g/cm2)
- Bone Mineral Density (BMD) g)
- Bone area (cm2)
- Tissue area (cm2)
- Fat tissue (mass and percentage) (g & %)
- Lean tissue (mass and percentage) (g & %)
- Total mass (g)
The iNSiGHT was designed to minimize the ionizing radiation (0.66 mGy) required to generate images and data. This minimization prevents any effect of the image acquisition on the subject, allowing for multiple imaging time points over the course of a longitudinal study.
Adjustable field of view and resolution
The iNSiGHT system has several levels of magnification, adjust the field of view (FOV) and resolution to suite your specific needs.
(100µm – up to 31µm in Digital Radiography Mode)
Models & Specifications
Scan Method : Cone Beam
Animal Size : Animal Size
Image Area (maximum) : 16.5 x 25.5cm
Pixel Size : 100µm at 1.2X 31µm at 4X with DR Mode
Operating System : Windows 10, 64bit
Dimensions (WxDxH) : 66 x 61 x 113 cm
Power : 110/240VAC, 50/60Hz, 200VA
Operating Temp : 10 ~ 40oC
Metabolic disorders occur when the normal metabolic processes within the body are disrupted, either due to genetic abnormalities, or developed due to environmental conditions.
There is a very long list of metabolic disorders, but most common are diabetes, metabolic syndrome, obesity, etc. There are a large number of small animal models of these diseases which could benefit from DXA imaging to help elucidate the genetic, molecular, and biochemical pathways that are integral to these pathologies.
The iNSiGHT system can be used to assess the progression or regression of disease and body composition measurements in response to a therapeutic regime by measuring changes in fat and lean mass over time. The colorimetric image allows for the differentiation, by way of regions of interest, of visceral and subcutaneous fat, which may play a key role in further understanding these pathologies. In this example the researchers were monitoring changes in body composition using a diabetic mouse model. They noted a reduction in fat mass, lean mass, and bone mineral content in the diabetic mouse model compared to the control.
Kishi K, Goto M, Tsuru Y, Hori M. Noninvasive monitoring of muscle atrophy and bone metabolic disorders using dual-energy X-ray absorptiometry in diabetic mice. Exp Anim. 2022 Sep 15. doi: 10.1538/expanim.22-0097. Epub ahead of print. PMID: 36104204
DXA - Imaging Gallery
Publications & Articles
A Bone Morphogenetic Protein-2 (BMP-2) Load Scaffold for Recruiting and Harvesting of Stem- and Immune- Cells for Therapy: Focus on the Role of the iNSiGHT Dual Energy X-Ray Absorptiometry (D(E)XA) System in the Development and Optimization of Osteo-Organoids
A Bone Morphogenetic Protein-2 (BMP-2) Load Scaffold for Recruiting and Harvesting of Stem-
Iron Overload – The Complications with Body Composition – A Short Article Review
Iron Overload - The Complications with Body Composition Download Document Here A Short
Dual Energy X-Ray Absorptiometry (DXA or DEXA) versus echo magnetic resonance imaging (echo MR): A short case study to compare body composition data collected using these instrumentations
Dual Energy X-Ray Absorptiometry (DXA or DEXA) versus echo magnetic resonance imaging (echo