This team of researchers in the Biology Department at Western University are investigating metabolic ways to increase efficiency of reprogramming adult cells to iPSCs. Embryonic stem cells are pluripotent, which means they can turn into any cell type found in the adult body and are therefore critical for human development1. Stem cells show great promise in
What oxygen levels are you using in your cellular research? Are your cells under hyperoxia, normoxia, hypoxia, or are you unsure? This free webinar hosted by Scintica Instrumentation provided our audience with an overview on the effects of oxygen during in vitro cell culturing. Further, the discussion focused on how to accurately
Since the first CO2 incubators were made commercially available in the late 1960’s they have become a staple for researchers to grow and maintain cells in vitro. These chambers have been highly effective for this task; however, they do not take into account one significant factor - oxygen. Currently, there is a push from
In the last year, I’ve spoken to countless hypoxia researchers in person, at conferences, and over the phone. When I reach out to these people the one question I always ask is “in what units does your current chamber report measured oxygen content?” to which I almost always get a resounding answer of percentage
The review article “Frequently asked questions in hypoxia research” by Wenger et al. covers several issues every researcher performing In vivo or In vitro hypoxia-based research should think about before performing their studies. This article is meant to help those in the early stages of attempting hypoxic studies. It should also be useful to
