Scientists out of Vanderbilt and the University of North Carolina at Chapel Hill have established two separate organoid lines derived from active Kras-induced mouse stomach tissue. These organoid lines embody an in vitro system displaying characteristics of abnormal cells within the tissue known as dysplasia. The study published in Nature Communications provides further insights
Externally nothing was altered. The HypoxyLab continues to be a well designed, compact, and ergonomically friendly hypoxia workstation perfect for any lab bench. All the changes themselves revolve around a complete redesign of the inner turret which houses the gas distribution system, temperature regulation, various sensors and HEPA filter. What does this mean for
Overview: In this free webinar, CytoSMART has partnered with prominent labs from academia to facilitate an open discussion about the use of live-cell imaging for viability analysis. We will be covering: The use of both brightfield and fluorescent live-cell imaging to analyze the effect of a treatment on cell viability. How cell viability
Chronic blood pressure recordings from the abdominal aorta are commonplace in cardiovascular physiology, however the surgical implantation of catheters can be challenging. This webinar provided an overview of the surgical protocol that we recommend when implanting Kaha Sciences blood pressure telemeters. We also discussed some simple strategies to avoid common problems and the
Carbon paste electrodes connected to a potentiostat have been shown to provide stable measurement of tissue oxygen levels in vivo. Now wireless telemetry from Kaha Sciences allows the chronic long-term measurement of tissue oxygen in rats living in their home cages. This webinar discussed how tissue oxygen measurements are made using carbon paste electrodes, how
Dr Pratik Thakkar from the University of Auckland presented data from their recent Hypertension publication where they used Cohousing mode (two telemeters in a single rat) to simultaneously record ICP, BP and brain tissue oxygen in a rat model of ischemic stroke. Spontaneously hypertensive rats were instrumented with telemeters to record BP, intracranial pressure,
Telemetry allows the long-term collection of data over days, weeks or months from animals in their home cages. Performing chronic studies presents a number of opportunities in terms of experimental design but also results in the collection of very large data sets. Large data sets come with challenges for the collection and analysis of
Ensuring you collect the best and most physiologically accurate data from your chronic telemetry experiments requires careful planning and experimental design. This webinar gave an insight into the practical aspects of designing chronic animal experiments to set you on the best path for success. We discussed the benefits of chronic studies, how to select
The mechanisms involved in the progression of heart failure includes cardiomyocyte death, initiation of the inflammatory response, and subsequent formation of a fibrotic scar. For this reason, diverse experimental models, such as cultured cardiac cells, isolated hearts, and animal models, have been created to mimic human myocardial infarction and heart failure. In a 2012
