(June 2, 2020) WEBINAR: A Roadmap to Physoxic Research: Where to Start and What to Consider
This free webinar hosted by Scintica Instrumentation provided researchers with an overview on the effects of oxygen during in vitro cell culturing. Further, the discussion focused on how to accurately determine and regulate the O2 levels during experiments. Last,we took researchers through our extensive list of hypoxia/physoxia options and guide them down the path from small to large scale chambers while providing helpful advice along the way.
Winners of the 2019 Nobel Prize in medicine figured out how our cells adapt to oxygen levels and have fully brought this to the forefront of all cellular research. Oxygen influences multiple physiological parameters within cells. As such, oxygen concentration should at least be considered when planning cell culture studies. In addition to monitoring and controlling for CO2, humidity and temperature, scientists should consider oxygen levels. Assessing oxygen concentration for all in vitro studies improves both scientific reproducibility and relevant outcomes by removing a potential confounding factor.
This webinar builds on these concepts and discussed the importance of oxygen in cell culture. It also provided a brief summary of relevant scientific publications. Participants gained a better understanding of how accurate oxygen regulation will contribute to improved reproducibility of their experimental work. Finally, participants learned about the different options that Scintica provides and questions to ask themselves as they begin to think about taking their research in this direction.
Topics discussed in this webinar included:
The meaning of hyperoxic, normoxic and hypoxic conditions at the cellular level
How hyperoxic conditions affect the HIF1 transcription factor and its many downstream effects
How oxygen is playing a larger and larger role in cellular labs
The options available to researchers from small to large scale and some considerations they may want to make
Justin Croft MSc – Justin studied behaviour and neuro-biology at The University of Western Ontario. His experience is wide ranging as he has an extensive background in genetics, microbiology, behaviour and neurology. Currently, Justin is the lead application specialist of the molecular division at Scintica Instrumentation, specializing in the fields of cancer, stem cells, microscopy and hypoxia.